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渗透胁迫诱导的小麦叶片细胞程序性死亡

林久生, 王根轩

兰州大学干旱农业生态国家重点实验室.甘肃,兰州 730000

摘要

用20% PEG6000(-0.63 MPa)溶液对小麦(Triticum aestivum)根系进行渗透胁迫,在DNA琼脂糖凝胶电泳图谱上观察到明显的梯状DNA条带,表明PEG处理诱发了DNA核小体间的断裂,从而表现出典型的细胞程序性死亡的生化特征;末端脱氧核糖核酸转移酶介导的3^-OH末端标记法(terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling,TUNEL)检测出现阳性结果。这些结果表明在PEG诱导小麦叶片死亡过程中,有一个阶段存在明显的细胞程序性死亡过程。结果同时表明,在小麦叶片处于程序性死亡时期,蛋白质含量、叶绿素含量的下降和电解质泄漏率的增加都比处于坏死时期的慢。

关键词： 渗透胁迫; 小麦叶片; 细胞程序性死亡; DNA梯状条带; TUNEL

Programmed cell death induced by osmotic stress in wheat leaves

Lin Jiusheng, Wang Genxuan

State Key Laboratory of Arid Agroecology,Lanzhou University.Lanzhou 730000,Gansu

Abstract

These results indicated that osmotic stress could induce PCD in wheat leaves. During PCD, the decrease in chlorophyll and protein contents and the increase in electrolyte leakage were all slower than those during necrosis.

Key words: Osmotic stress;Wheat leaves;Programmed cell death;DNA ladder;TUNEL

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引用本文：

林久生, 王根轩. 渗透胁迫诱导的小麦叶片细胞程序性死亡[J]. 生理学报 2001; 53 (3): .

Lin Jiusheng, Wang Genxuan. Programmed cell death induced by osmotic stress in wheat leaves. Acta Physiol Sin 2001; 53 (3): (in Chinese with English abstract).