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细胞外钙调素对烟草悬浮培养细胞蛋白质磷酸化作用的影响

张来群, 李芳, 孙大业

河北师范大学生命科学学院.河北,石家庄 050016

摘要

烟草细胞细胞质蛋白质磷酸化活性在细胞培养过程中逐渐增加,达到最高峰后又开始下降。在细胞质蛋白质磷酸化强度高峰时,加入抗CaM血清后,细胞质蛋白质磷酸化活性受到了部分抑制。加抗CaM血清后再补加CaM能够部分解除抗CaM血清对细胞质部分与细胞核部分蛋白质磷酸化的抑制作用。外加纯化钙调素可以引起烟草悬浮培养细胞细胞质蛋白质磷酸化的活性增强,并且这种增强作用具有时间(高峰为70min)与剂量(最适为CaM 10~(-7) mmol/L)依赖性。CaM引起的细胞质蛋白质磷酸化变化与红光所引起的细胞质蛋白质磷酸化变化在时间进程上是不相同的。

关键词： 烟草悬浮培养细胞; 细胞外钙调素; 蛋白质磷酸化

The effects of extracellular calmodulin on protein phosphorylation in cytoplasmic fraction from suspension-cultured tobacco (Nicotiana tobacum L.) cells

Zhang Laiqun, Li Fang, Sun Daye

Institute of Molecular Biology,Hebei Normal University.Shijiazhuang 050016,Hebei

Abstract

The effects of extracellular calmodulin on the activity of protein phosphorylation were studied in suspension-cultured tobacco cells. The activity of protein phosphorylation in cytoplasmic fraction increased gradually as measured in different culture days, as it reaches peak then decrease. On its peak day, the activityof protein phosphorylation could be blocked by the anti-CaM serum, and addition of exogenous purified CaM could eliminate the inhibitory effects of anti-CaM serum. The activity of protein phosphorylation of cytoplasmic fraction from suspension-cultured tobacco cells was also activated by pure CaM. The activation was time- and dose-dependent. The extracellular CaM induced activation of protein phosphorylatino was different from red light induced one with respect to their time courses.

Key words: Suspension cultured tobacco cells;Extracellular CaM;Protein phosphorylation

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引用本文：

张来群, 李芳, 孙大业. 细胞外钙调素对烟草悬浮培养细胞蛋白质磷酸化作用的影响[J]. 生理学报 2001; 53 (3): .

Zhang Laiqun, Li Fang, Sun Daye. The effects of extracellular calmodulin on protein phosphorylation in cytoplasmic fraction from suspension-cultured tobacco (Nicotiana tobacum L.) cells. Acta Physiol Sin 2001; 53 (3): (in Chinese with English abstract).