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#beta#-淀粉样蛋白31-35片段对海马神经元分离膜片Ca~(2+)激活大电导钾通道的抑制

祁金顺, 乔健天

山西医科大学神经生物学研究室.山西,太原 030001

摘要

为了确定#beta#-淀粉样蛋白(A#beta#P)在影响神经元电生理特性并导致神经毒作用时的最短活性序列,实验采用膜片钳技术,在急性分离的大鼠海马CA1区锥体细胞的“内面向外”式膜片上,观察了A#beta#P的31-35和25-35片段对Ca~(2+)激活大电导钾(BK)通道活动的影响。

关键词： β-淀粉样蛋白; AβP31-35; AβP25-35; Ca2+激活大电导钾通道; 海马神经元; 膜片钳

Suppression of large conductance Ca~(2+)-activated K~(+) channels by amyloid #beta#-protein fragment 31-35 in membrane patches excised from hippocampal neurons

Qi Jinshun, Qiao Jiantian

Department of Neurobiology,Shanxi Medical University.Taiyuan 030001,Shanxi

Abstract

To clarify the shortest essential active sequence in amyloid #beta#-protein (A#beta#P) responsible for affecting neuronal electrophysiological properties, the efffects of fragments 31-35 and 25-35 of A#beta#P on the arge conductance Ca~(2+)-activated potassium (BK) channels were investigated in the "inside-out" membrane patches excised from hippocampal neurons of rats.

Key words: Amyloid #beta#-protein;Fragment 31-35;Fragment 25-35;BK channel;Hippocampal neuron;Membrane patch

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引用本文：

祁金顺, 乔健天. #beta#-淀粉样蛋白31-35片段对海马神经元分离膜片Ca~(2+)激活大电导钾通道的抑制[J]. 生理学报 2001; 53 (3): .

Qi Jinshun, Qiao Jiantian. Suppression of large conductance Ca~(2+)-activated K~(+) channels by amyloid #beta#-protein fragment 31-35 in membrane patches excised from hippocampal neurons. Acta Physiol Sin 2001; 53 (3): (in Chinese with English abstract).