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乙酰胆碱和A23187对离体大鼠肾上腺髓质细胞肾上腺素分泌的作用

刘懿, 凌诒萍, 俞彰, 吴正泉, 钟慈声

复旦大学基础医学院生物物理学教研室.上海 200032

摘要

通过胆碱能激动剂乙酰胆碱及离子诱导剂A23187(以下简称激动剂)作用于分离的肾上腺髓质细胞,以引起离体细胞的刺激-分泌耦联过程。运用细胞立体形态计量法计算分泌过程中的嗜铬颗粒数目的变化,运用电镜X射线显微分析法测量分泌过程中嗜铬颗粒内钙含量的变化,并运用高压液相色谱分析法测定离体细胞在激动剂作用后的肾上腺素分泌情况。

关键词： 离体肾上腺髓质细胞; 嗜铬颗粒; 颗粒数密度; 钙含量; 肾上腺素

Acetylcholine and A23187 stimulate epinephrine secretion of isolated rat adrenal medulla cells in vitro

Liu Yi, Ling Yiping, Yu Zhang, Wu Zhengquan, Zhong Cisheng

Department of Biophysics,School of Basic Medical Sciences,Fudan University.Shanghai 200032

Abstract

Acetylcholine and A23187, two acetylcholinergic agonists, were used to induce the stimulus-secretion coupling of the isolated rat adrenal medulla cells. Morphometry of numerical density and electron probe X-ray quantitative microanalysis were adopted to measure are alterations in the number and calcium content of chromaffin granules in adrenal medulla cells during the agonist treatment. Secretion of epinephrine was detected by the high performance liquid chromatography (HPLC).

Key words: Isolated adrenal medulla cell;Chromaffin granule;Numerical density;Calcium content;Epinephrine

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引用本文：

刘懿, 凌诒萍, 俞彰, 吴正泉, 钟慈声. 乙酰胆碱和A23187对离体大鼠肾上腺髓质细胞肾上腺素分泌的作用[J]. 生理学报 2001; 53 (2): .

Liu Yi, Ling Yiping, Yu Zhang, Wu Zhengquan, Zhong Cisheng. Acetylcholine and A23187 stimulate epinephrine secretion of isolated rat adrenal medulla cells in vitro. Acta Physiol Sin 2001; 53 (2): (in Chinese with English abstract).