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缺血引起沙土鼠纹状体DARPP-32磷酸化状态的变化

孙亚锋, 唐放鸣, 王然, 张光毅, 金国章

徐州医学院生物化学与分子生物学研究中心.江苏,徐州 221002；中科院上海药物研究所.上海 200031

摘要

短暂性缺血纹状体DARPP-32的免疫学活性和蛋白含量无明显改变。在缺血10min内,随缺血时间的延长,体外DARPP-32的[~(32)P]的掺入量在缺血5min时升高,在缺血2、7、10min时均降低,而反向磷酸化的测定结果表明体内DARPP-32磷酸化水平增高,说明缺血可诱导DARPP-32磷酸化水平变化。

关键词： 脑缺血; 蒙古沙土鼠; DARPP-32; 反向磷酸化; Westernblotting

Changes in the state of phosphorylation of DARPP-32 of gerbil striatum during ischemia

Sun Yafeng, Tang Fangming, Wang Ran, Zhang Guangyi, Jin Guozhang

Research Center of Biochemistry and Molecular Biology,Xuzhou Medical College.Xuzhou 221002,Jiangsu；China

Abstract

Transient forebrain ischemia had no effect on the immunoreaction of DARPP-32 in striatum, but the state of phosphorylation of DARPP-32 showed a significant change. The [~(32)P] phosphate incorporation in DARPP-32 decreased after 2, 7 or 10 min of ischemia, but increased after a 5-min ischemia. In contrast, the result of back-phosphorylation in vivo was opposite to that obtained in vitro.

Key words: Cerebral ischemia;Mongolian gerbil;DARPP-32;Back phosphoiylation;Striatum

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引用本文：

孙亚锋, 唐放鸣, 王然, 张光毅, 金国章. 缺血引起沙土鼠纹状体DARPP-32磷酸化状态的变化[J]. 生理学报 2001; 53 (2): .

Sun Yafeng, Tang Fangming, Wang Ran, Zhang Guangyi, Jin Guozhang. Changes in the state of phosphorylation of DARPP-32 of gerbil striatum during ischemia. Acta Physiol Sin 2001; 53 (2): (in Chinese with English abstract).