ISSN 0371-0874, CN 31-1352/Q

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哈姆林甜橙与粗柠檬体细胞杂种的育性

伊华林, 邓秀新

华中农业大学作物遗传改良国家重点实验室.湖北,武汉 430070

摘要

对异源四倍体柑桔体细胞杂种“哈姆林甜橙+粗柠檬”及其亲本的花粉活力、花器官发育、花器官形态发生与花粉母细胞减数分裂四分体阶段进行了观察和统计。结果发现:“哈姆林甜橙+粗柠檬”的花粉染色活力、萌发率、每花药中花粉粒数均居于其双亲之间,花器官发育及其形态发生具有双亲的特点。但小花粉及花粉母细胞减数分裂过程中形成的不正常四分体比率远远高于其双亲。以体细胞杂种“哈姆林甜橙+粗柠檬”为花粉亲本,与二倍体单胚类型宜昌橙与华农本地早的有性后代杂交,获得了110棵有性后代植株,其中三倍体82棵,二倍体和其它倍性的植株28棵。

关键词: 柑桔; 体细胞杂种; 育性; 有性杂交; 倍性

Fertility of Hamlin sweet orange and rough lemon somatic hybrid

Yi Hualin, Deng Xiuxin

National Key Laboratory of Crops Genetic Improvement,Huazhong Agricultural University.Wuhan 430070,Hubei

Abstract

The pollen viability, the development and morphogenesis of flower organ, tetradstage during meiosis of allotetraploid citrus somatic hybrid "Hamlin sweet orange (Citrus sinensis)+Rough Lemon (C. jambhiri)" and those of its parent lines were studied.

Key words: Citrus;Somatic hybrid;Fertility;Sexual hybridization;Ploidy

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引用本文:

伊华林, 邓秀新. 哈姆林甜橙与粗柠檬体细胞杂种的育性[J]. 生理学报 2001; 53 (2): .

Yi Hualin, Deng Xiuxin. Fertility of Hamlin sweet orange and rough lemon somatic hybrid. Acta Physiol Sin 2001; 53 (2): (in Chinese with English abstract).