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秋水仙碱对大麦离体培养小孢子存活与成苗的影响

陆瑞菊, 黄剑华, 孙月芳, 王亦菲, 周润梅

上海市农业科学院生物技术研究中心上海市农业遗传育种重点实验室.上海 201106

摘要

以4份大田生长的优良大麦品种/品系为材料,采用超速旋切法分离小孢子进行培养。提取液和预处理液中添加适当浓度的秋水仙碱可明显提高大麦小孢子的存活率、胚状体的成苗潜力。

关键词： 大麦; 小孢子培养; 秋水仙碱; 胚状体; 绿苗分化率

The effect of colchicine on survival and regeneration of isolated microspores in barley

Lu Ruiju, Huang Jianhua, Sun Yuefang, Wang Yifei, Zhou Runmei

Shanghai Key Laboratory of Agricultural Genetics and Breeding,Agro-Biotech Research Center,Shanghai Academy of Agricultural Sciences.Shanghai 201106

Abstract

The effects of colchcine on the survival percentage and on the regeneration frequency were tested in culture microspore from 4 cultivars of barley.

Key words: Barley;Microspores culture;Colchicine;Embryogenesis;Green plant regeneration

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引用本文：

陆瑞菊, 黄剑华, 孙月芳, 王亦菲, 周润梅. 秋水仙碱对大麦离体培养小孢子存活与成苗的影响[J]. 生理学报 2001; 53 (2): .

Lu Ruiju, Huang Jianhua, Sun Yuefang, Wang Yifei, Zhou Runmei. The effect of colchicine on survival and regeneration of isolated microspores in barley. Acta Physiol Sin 2001; 53 (2): (in Chinese with English abstract).