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顽拗性黄皮种子脱水过程中活性氧清除酶活性的变化

王艳, 李韶山, 何军贤, 傅家瑞

暨南大学生命科学和技术学院.广东,广州 510632；华南师范大学生命科学学院.广东,广州 510631；四川大学生物系.四川,成都 610064；中山大学生命科学学院.广东,广州 510275

摘要

以正常性种子花生为对照,研究了顽拗性黄皮种子脱水过程中活性氧清除酶、膜脂过氧化作用以及电解质渗漏率的变化。随着含水量的下降,黄皮胚的电解质渗漏率和膜脂过氧化产物丙二醛(MDA)含量均显著增加;当黄皮胚含水量下降至40%后,SOD活性开始急剧下降,而POD和CAT活性在胚含水量下降过程中呈现出缓慢下降的趋势。花生胚在含水量从45%降至14%的过程中,电解质渗漏率没有明显增加,MDA含量只有少量增加;当含水量降至14%后,电解质渗漏率出现少量增加。花生胚脱水初期,活性氧清除酶活性明显增加,并在整个脱水过程中维持较高的水平。以上结果表明:顽拗性种子黄皮的脱水敏感性与活性氧清除酶相对活性变化有关。脱水引起黄皮胚活性氧清除酶活性降低,活性氧清除能力下降,膜脂过氧化作用加强,膜透性增大。黄皮胚的膜系统可能是脱水伤害的靶位之一。

关键词： 黄皮; 脱水敏感性; 脂质过氧化; 活性氧清除酶; 顽拗性种子

Changes in activity of reactive-oxygen-scavenging enzymes in recalcitrant wampee (Clausena lansium) seeds duing desiccation

Wang Yan, Li Shaoshan, He Junxian, Fu Jiarui

School of Life Science and Technology,Jinan University.Guangzhou 510632,Guangdong；China；Department of Biology,Sichuan University.Chengdu 610064,Sichuan

Abstract

The activity of reactive-oxygen-scavenging enzymes, membrane lipid peroxidation and electrolyte leakage in embryos of wampee seeds during desiccation were measured in order to investigate the physiology of desiccation-sensitivity in recalcitrant seeds.

Key words: Clausena lansium;Desiccation sensitivity;Lipid peroxidation;Reactive-oxygen-scavenging enzymes;Recalcitrant seeds

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引用本文：

王艳, 李韶山, 何军贤, 傅家瑞. 顽拗性黄皮种子脱水过程中活性氧清除酶活性的变化[J]. 生理学报 2001; 53 (1): .

Wang Yan, Li Shaoshan, He Junxian, Fu Jiarui. Changes in activity of reactive-oxygen-scavenging enzymes in recalcitrant wampee (Clausena lansium) seeds duing desiccation. Acta Physiol Sin 2001; 53 (1): (in Chinese with English abstract).