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大豆叶片衰老过程中质膜蛋白激酶自磷酸化状态和催化活性的变化

王宁宁, 王勇, 张自立, 朱亮基, 张韧

南开大学生物化学与分子生物学系.天津 300071；Department of Biological Science,University of Wollongong.NSW 2522,Austrlia

摘要

以大豆幼苗初生叶为材料研究了衰老过程中质膜蛋白激酶自磷酸化状态和催化活性的变化。结果发现质膜上一个57kD的蛋白激酶分子上有多个自磷酸化位点,而且自磷酸化反应能提高该酶催化组蛋白H1磷酸化的激酶活力。进一步的研究表明诱导衰老处理造成的57kD蛋白激酶自磷酸化状态的变化,可能对调节它在衰老过程中催化活性的变化起重要作用;而外源6-BA预处理则能够维持57kD蛋白激酶体内高自磷酸化状态,保持该激酶在衰老过程中的催化活力。对衰老和6-BA处理过程中质膜上39和47kD蛋白激酶自磷酸化状态变化的研究表明,这两种激酶可能参与大豆叶片对6-BA刺激信号的传导和/或应答反应过程。

关键词： 大豆; 叶片; 细胞分裂素; 蛋白激酶; 自磷酸化反应

Senescence-induced changes in autophosphorylation status and catalytic activity of plasma membrane protein kinases in soybean primary leaves

Wang Ningning, Wang Yong, Zhang Zili, Zhu Liangji, Zhang Ren

Department of Biochemistry and Molecular Biology,Nankai University.Tianjin 300071；Australia

Abstract

The results suggested that there were multiple sites on a 57 kD calcium-dependent protein kinase (CDPK) molecule for autophosphorylation and that autophosphorylation could improve its catalytic activity in phosphorylating histone H1. ST-induced changes in in vivo autophosphorylation status of the 57 kD CDPK may play an important role in regulating its catalytic activity during leaf senescence, and 6-BA pretreatment may make the high catalytic activity of this kinase longlasting. The effect of 6-BA application on ST-induced changes of autophosphorylation activity of a 39 kD and a 47 kD protein kinases implied involvement of these two kinases in cytokinin stimulus/response cascade system.

Key words: Soybean;Leaf;Cytokinin;Protein kinase;Au-tophosphorylation

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引用本文：

王宁宁, 王勇, 张自立, 朱亮基, 张韧. 大豆叶片衰老过程中质膜蛋白激酶自磷酸化状态和催化活性的变化[J]. 生理学报 2001; 53 (1): .

Wang Ningning, Wang Yong, Zhang Zili, Zhu Liangji, Zhang Ren. Senescence-induced changes in autophosphorylation status and catalytic activity of plasma membrane protein kinases in soybean primary leaves. Acta Physiol Sin 2001; 53 (1): (in Chinese with English abstract).