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外源~(1)O_(2)和·OH及其清除剂对愈伤组织交替途径发生与运行的影响

周功克, 孔英珍, 李红玉, 梁厚果

兰州大学干旱农业生态国家重点实验室.甘肃,兰州 730000；兰州大学生命科学学院.甘肃,兰州 730000

摘要

外源~(1)O_(2)和·OH处理继代培养14d的烟草愈伤组织24h后,愈伤组织内的交替途径的实际运行显著上升,但对交替途径容量影响不大;而经·OH处理后的愈伤组织交替途径容量和实际运行均明显地受到抑制,但交替途径的实际运行对·OH更加敏感。活性氧产生系统中加入相关清除剂进行实验,得到与此相对应的结果:~(1)O_(2)的清除剂His处理能明显地降低或抑制~(1)O_(2)所增加的交替途径实际运行量,但对交替途径容量则几乎均无影响,而用·OH的清除剂DMSO和MAN分别处理愈伤组织后,DMSO和MAN均能解除·OH对交替途径容量和实际运行的抑制。这些结果表明,~(1)O_(2)诱导烟草愈伤组织交替途径的实际运行,但对其容量的作用不大,而·OH则明显地抑制交替途径容量和实际运行。推测~(1)O_(2)和·OH对抗氰途径的影响可能是通过对AOX活性的调节。

关键词： 烟草愈伤组织; ·OH.1O2; 交替途径

Effects of exogenous ~(1)O_(2) and ·OH and their scavengers on the capacity and operation of the alternative respiratory pathway in tobacco callus

Zhou Gongke, Kong Yingzhen, Li Hongyu, Liang Houguo

The State Key Laboratory of Arid Agroecology,Lanzhou University.Lanzhou 730000,Gansu；China

Abstract

In tobacco (Nicotiana rustica L. Gansu Yellow Flower) callus, addition of exogenous ~(1)O_(2) induced a pronounced increase in the operation activity of alternative respiratory pathway without affecting its capacity. The operation activity of alternative pathway activated by ~(1)O_(2) can be abolished by its scavenger (His). Exogenous ·OH diminished the operation activity and capacity of alternative pathway, which could be recovered by the addition of ·OH scavengers, such as DMSO or MAN. These observations suggest that ~(1)O_(2) is involved in some way in the activating the alternative pathway, but not in the induction of the capacity of alternative pathway; but ·OH can suppress both the actual operation activity and capacity of alternative pathway remarkably. We deduced that ~(1)O_(2) and ·OH might affect the alternative pathway by regulating the activity of the alternative oxidase.

Key words: Tobacco callus;.OH，O_(2);Alternative respiratory path way

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引用本文：

周功克, 孔英珍, 李红玉, 梁厚果. 外源~(1)O_(2)和·OH及其清除剂对愈伤组织交替途径发生与运行的影响[J]. 生理学报 2001; 53 (1): .

Zhou Gongke, Kong Yingzhen, Li Hongyu, Liang Houguo. Effects of exogenous ~(1)O_(2) and ·OH and their scavengers on the capacity and operation of the alternative respiratory pathway in tobacco callus. Acta Physiol Sin 2001; 53 (1): (in Chinese with English abstract).