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渗透胁迫对山黧豆幼苗H_(2)O_(2)及毒素积累的影响

邢更生, 周功克, 李志孝, 崔凯荣

兰州大学干旱农业生态国家重点实验室.甘肃,兰州 730000；兰州大学应用有机化学国家重点实验室.甘肃,兰州 730000

摘要

用PEG经根部处理15d龄的山黧豆幼苗,取幼苗叶片为实验材料,测定过氧化氢酶(CAT)、过氧化物酶(POD)活性、过氧化氢(H_(2)O_(2))和毒素(#beta#-N-oxalyl-#alpha#,#beta#-diaminopropionic acid,ODAP)的含量。结果表明,随着PEG处理时间的延长,POD和CAT活性降低,而H_(2)O_(2)和ODAP含量显著升高;在PEG处理液中加入二乙基二硫代氨基甲酸钠(diethyldithiocarbamate,DDC)和氨基三唑(aminotriazole,AT)后分别抑制和促进H_(2)O_(2)的产生,DDC可降低叶片中ODAP的含量,AT则使ODAP积累。由此我们推测,水分胁迫条件下活性氧代谢与ODAP的积累有关。

关键词： 渗透胁迫; 山黧豆; 过氧化氢; 毒素; 抗氧化酶

Effects of osmotic stress on accumulation of H_(2)O_(2) and ODAP in grass pea (Lathyrus sativus)

Xing Gengsheng, Zhou Gongke, Li Zhixiao, Cui Kairong

The State Key Laboratory of Arid Agroecology,Lanzhou University.Lanzhou 730000,Gansu；China

Abstract

Roots of fifteen-day-old grass pea seedlings were treated with PEG and then the leaves were used as material. Changes in activities of catalase and peroxidase, and hydrogen peroxide and #beta#-N-oxalyl-#alpha#, #beta#-diaminopropionic acid (ODAP) contents during PEG treatment, were assayed. The results indicated that during PEG treatment, activities of peroxidase and catalase decreased, and hydrogen peroxide and ODAP content increased greatly. Diethyldithiocarbamate or aminotriazole strongly decreased or increased respectively the H_(2)O_(2) content. While the accumulation of ODAP was decreased markedly by diethyldithiocarbamate, it was increased significantly by aminotriazole. Therefore, we suggest that the accumulation of ODAP might be related to the increase in H_(2)O_(2) content under osmotic stress.

Key words: Osmotic stress;Lathyrus sativus;Hydrogen peroxide;#beta#- N-oxalyl-#alpha#;#beta#-diaminopropionic acid(ODAP)

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引用本文：

邢更生, 周功克, 李志孝, 崔凯荣. 渗透胁迫对山黧豆幼苗H_(2)O_(2)及毒素积累的影响[J]. 生理学报 2001; 53 (1): .

Xing Gengsheng, Zhou Gongke, Li Zhixiao, Cui Kairong. Effects of osmotic stress on accumulation of H_(2)O_(2) and ODAP in grass pea (Lathyrus sativus). Acta Physiol Sin 2001; 53 (1): (in Chinese with English abstract).