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基因枪法转化小麦的金粉用量及转基因埴株表型特征分析

安海龙, 卫志明, 黄健秋

中科院上海植物生理研究所.上海 200032

摘要

小麦幼胚瞬间转化的金粉用量以每枪500#mu#g为佳。对获得的T_(0)及T_(1)代植株的PCR及Southern分析证实了外源bar基因片段已经稳定整合至小麦基因组中;对T_(1)代植株的L-PPT涂抹实验表明转化植株对该除草剂的抗性水平较低;分析了T_(0)、T_(1)及T_(2)代植株的表型特征,结果表明T_(0)代植株生长矮小、结实率低等表型方面的异常很可能只是一种由于生理及栽培原因所导致的暂时现象。

关键词： 基因枪; 小麦; 遗传转化; 表型特征

Gold particle amount per bombardment of wheat transformation by biolistic method and phenotypic character analysis of transgenic plants and their progenes

An Hailong, Wei Zhiming, Huang Jianqiu

Shanghai Institute of Plant Physiology,Chinese Academy of Sciences.Shanghai 200032

Abstract

Effects of gold particle amount pe bombardment (30, 100, 250, 500 #mu#g) on transient and stable transformation of immature wheat embryos, were studied. And 500 #mu#g gold particle per bombardment is superior to others both in GUS transient expression and transgenic plant production. PCR and Southern analyses of T_(0) and T_(1) plants confirmed the integration of foreign bar gene fragments into wheat genomes. T_(1) plants smeared with L-PPT 200 mg/L showed a low level of resistance to this herbicide. The comparison of phenotypic character of T_(0), T_(1), T_(2) and their control plants showed that the phenotype abnormality of T_(0) plants is only a temporary phenomenon due to physiological and cultivated conditions, and the plants will recover from this abnormality partially or fully in the following progenes.

Key words: Particle bombardment;Triticum aestivum L.;Genetic transformation;Phenotypic character

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引用本文：

安海龙, 卫志明, 黄健秋. 基因枪法转化小麦的金粉用量及转基因埴株表型特征分析[J]. 生理学报 2001; 53 (1): .

An Hailong, Wei Zhiming, Huang Jianqiu. Gold particle amount per bombardment of wheat transformation by biolistic method and phenotypic character analysis of transgenic plants and their progenes. Acta Physiol Sin 2001; 53 (1): (in Chinese with English abstract).