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心室成纤维细胞条件培养液促进成纤维细胞胶原合成和增殖

龚素珍, 刘培庆, 鲁伟, 王庭槐, 符史干, 潘敬运

中山医科大学生理学教研室.广东,广州 510089

摘要

心室成纤维细胞条件培养液(FCGM)能明显增加细胞自身的[~(3)H]-proline的掺入率和[~(3)H]-TdR的掺入率,并具有剂量依赖性;FCGM也能促进细胞自身c-fos基因的表达,刺激后1h达高峰。ET_(A)受体拮抗剂BQ_(123)能部分阻断FCGM增加成纤维细胞胶原合成和增殖作用,而AT_(1)受体拮抗剂CV11974和#alpha#-肾上腺素受体拮抗剂regitin无此效果。

关键词： 心室成纤维细胞; [3H］-脯氨酸; ［3H］-胸腺嘧啶核苷; c-fos mRNA; 心脏

Role of conditioned growth medium for ventricular fibroblasts in promoting fibroblast collagen synthesis and proliferation

Gong Suzhen, Liu Peiqing, Lu Wei, Wang Tinghuai, Fu Shigan, Pan Jingyun

Department of Physiology,Sun Yat-Sen University of Medical Sciences.Guangzhou 510089,Guangdong

Abstract

Ventricular fibroblasts were cultured using conditioned growth medium for ventricular fibroblasts (FCGM). The rate of the total collagen synthesis of ventricular fibroblasts was measured by assaying the incorporation rate of [~(3)H]-proline, whereas the proliferation of ventricular fibroblasts was assessed by determining the incorporation rate of [~(3)H]-TdR and the expression of c-fos genes.

Key words: ventricular fibroblast;［3H］-proline;［3H］-TdR;c-fos mRNA;Heart

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引用本文：

龚素珍, 刘培庆, 鲁伟, 王庭槐, 符史干, 潘敬运. 心室成纤维细胞条件培养液促进成纤维细胞胶原合成和增殖[J]. 生理学报 2001; 53 (1): .

Gong Suzhen, Liu Peiqing, Lu Wei, Wang Tinghuai, Fu Shigan, Pan Jingyun. Role of conditioned growth medium for ventricular fibroblasts in promoting fibroblast collagen synthesis and proliferation. Acta Physiol Sin 2001; 53 (1): (in Chinese with English abstract).