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鞘内注射NOS反义寡核苷酸抑制吗啡戒断大鼠脊髓和脑干NMDA_(1A)受体mRNA表达的增加

曹君利, 刘惠芬, 周文华, 杨国栋, 张励才, 曾因明

徐州医学院附属医院麻醉科.江苏,徐州 221002；宁波戒毒研究中心.浙江,宁波 315010；江苏省麻醉学重点实验室.江苏,徐州 221002

摘要

鞘内注射NOS反义寡核苷酸能明显减轻吗啡戒断反应,且脑型NOS (nNOS)反义寡核苷酸的作用强于内皮型NOS(eNOS)反义寡核苷酸。吗啡依赖大鼠脊髓和脑干NMDA_(1A)R mRNA表达增加,纳洛酮催促戒断,使其进一步增加;鞘内注射nNOS反义寡核苷酸,能明显抑制吗啡戒断大鼠脊髓和脑干NMDA_(1A)R mRNA表达的增加;eNOS反义寡核苷酸也可抑制吗啡戒断大鼠脊髓NMDA_(1A)R mRNA表达的增加,但作用弱于nNOS反义寡核苷酸,对脑干NMDA_(1A)R mRNA表达无明显影响。

关键词： 吗啡; 戒断; NO; NMDA

Intrathecal injection of NOS antisense oligonucleotides inhibits the increase of NMDA_(1A)R mRNA expression in the spinal cord and brainstem of morphine-withdrawal rats

Cao Junli, Liu Huifen, Zhou Wenhua, Yang Guodong, Zhang Licai, Zeng Yinming

Department of Anesthesiology,Affiliated Hospital of Xuzhou Medical College.Xuzhou 221002,Jiangsu；China；Jiangsu Province Key Laboratory of Anesthesiology.Xuzhou 221002,Jiangsu

Abstract

Methods of reverse transcription polymerase chain reaction (RT-PCR), intrathecal injection and antisense drugs were used to study the effects of nitric oxide (NO) on the scores of morphine-withdrawal syndrome and the expression of NMDA_(1A)R mRNA in rat spinal cord and brainstem. It is suggested that NO mediates morphine-withdrawal reaction and participates in modulating the expression of NMDA_(1A)R mRNA in morphine-withdrawal rats.

Key words: Morphine;withdrawal;NO;NMDA

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引用本文：

曹君利, 刘惠芬, 周文华, 杨国栋, 张励才, 曾因明. 鞘内注射NOS反义寡核苷酸抑制吗啡戒断大鼠脊髓和脑干NMDA_(1A)受体mRNA表达的增加[J]. 生理学报 2001; 53 (1): .

Cao Junli, Liu Huifen, Zhou Wenhua, Yang Guodong, Zhang Licai, Zeng Yinming. Intrathecal injection of NOS antisense oligonucleotides inhibits the increase of NMDA_(1A)R mRNA expression in the spinal cord and brainstem of morphine-withdrawal rats. Acta Physiol Sin 2001; 53 (1): (in Chinese with English abstract).