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Genistein对大鼠垂体前叶细胞增殖的抑制作用

张庆红, 胡玉珍, 周士胜, 王复周

第四军医大学基础部生理学教研室.陕西,西安 710032

摘要

结果显示:genistein作用48h后可明显抑制正常大鼠垂体前叶细胞和垂体瘤细胞株AtT-20增殖。流式细胞仪检测发现,50和100#mu#mol/L genistein可将AtT-20细胞阻断于G_(0)/G_(1)期及G_(2)/M期,并出现凋亡峰,凋亡率分别达19.9%和36.4%。电镜照片显示有调亡细胞。

关键词： genistein; 垂体; 增殖; 凋亡; 酪氨酸蛋白激酶抑制剂

Inhibitory effect of genistein on the proliferation of the anterior pituitary cells of rats

Zhang Qinghong, Hu Yuzhen, Zhou Shizhen, Wang Fuzhou

Department of Physiology,Fourth Military Medical University.Xi^an 710032,Shaanxi

Abstract

Genistein significantly inhibited the proliferation of rat anterior pituitary cells and mouse AtT-20 cells. Genistein (50 and 100 #mu#mol/L) blocked the proliferation of AtT-20 cells at G_(0)/G_(1) and G_(2)/M phases and evoked an apoptotic peak of these cells with an apoptotic ratio of 19.9% and 36.4%. The apoptotic cells were also observed under the electron microscope. In consequence, genistein, as a tyrosine kinase inhibitor, can significantly inhibit the proliferation of pituitary cells possibly by inducing apoptosis, and the tyrosine kinase activity may play a key role in the proliferation and differentiation of pituitary cells.

Key words: genistein;Pituitary;Proliferation;Apoptosis;tyrosine kinase inhibitor

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引用本文：

张庆红, 胡玉珍, 周士胜, 王复周. Genistein对大鼠垂体前叶细胞增殖的抑制作用[J]. 生理学报 2001; 53 (1): .

Zhang Qinghong, Hu Yuzhen, Zhou Shizhen, Wang Fuzhou. Inhibitory effect of genistein on the proliferation of the anterior pituitary cells of rats. Acta Physiol Sin 2001; 53 (1): (in Chinese with English abstract).