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流动剪切力对鼠脑微血管内皮细胞ICAM-1表达的影响

宋晓燕, 曾衍钧, 李彩霞, 廖东华, 胡金麟, 郝延磊

北京工业大学生物力学和医学信息研究所.北京 100022；解放军总医院微循环室.北京 100853；解放军总医院神经内科实验室.北京 100853

摘要

脑微血管内皮细胞在剪切力作用下ICAM-1的表达呈特异上调,且存在着时间依赖性,与一定范围内的剪切力强度无关。用对细胞施加剪切力作用后提取上清液孵育内皮细胞的方法证明:剪切力对鼠脑微血管内皮细胞ICAM-1表达的影响,是直接作用于内皮细胞引起的细胞内的直接反应,而不是剪切力导致细胞先释放细胞因子,释放的细胞因子再引起ICAM-1变化的间接反应。

关键词： 剪切力; 鼠脑微血管内皮细胞; 内皮细胞粘附分子

ICAM-1 expression of rat brain microvascular endothelial cells caused by fluid shear force

Song Xiaoyan, Zeng Yanjun, Li Caixia, Liao Donghua, Hu Jinlin, Hao Yanlei

Biomechanics and Medical Information Institute,Beijing Polytechnic University.Beijing 100022；China；Laboratory of Neuroscience,Chinese PLA General Hospital.Beijing 100853

Abstract

We studied the effect of shear stress (SS) on intercellular adhesion molecule-1 (ICAM-1) expression of rat brain microvascular endothelial cells (RBMECs) using the parallel plate flow chamber method. It was demonstrated that RBMECs showed a time-dependent, but not a force-dependent, upregulation in ICAM-1 expression. Endothelial cell surface expression of ICAM-1 in the supernatants of RBMECs exposed to SS was not changed, thus excluding the possibility that the upregulated expression is due to the factors synthesized by these cells.

Key words: shear force;RBMECs;ICAM-1

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引用本文：

宋晓燕, 曾衍钧, 李彩霞, 廖东华, 胡金麟, 郝延磊. 流动剪切力对鼠脑微血管内皮细胞ICAM-1表达的影响[J]. 生理学报 2001; 53 (1): .

Song Xiaoyan, Zeng Yanjun, Li Caixia, Liao Donghua, Hu Jinlin, Hao Yanlei. ICAM-1 expression of rat brain microvascular endothelial cells caused by fluid shear force. Acta Physiol Sin 2001; 53 (1): (in Chinese with English abstract).