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17#beta#-雌二醇诱导血管内皮细胞一氧化氮释放及其与细胞内钙的关系

王庭槐, 杨丹, 刘培庆, 龚素珍, 鲁伟, 潘敬运

中山医科大学生理教研室.广东,广州 510089

摘要

利用小牛胸主动脉内皮细胞(BAECs)作为模型,观察17#beta#-雌二醇(E_(2))对BAECs一氧化氮(NO)释放、一氧化氮合酶(eNOS)mRNA表达和细胞内钙([Ca~(2+)]_(i))的影响,以及雌激素受体(ER)拮抗剂tamoxifen和NOS抑制剂(L-NAME)的作用。提示E_(2)能促进BAECs的NO释放和eNOS mRNA表达,该作用至少部分通过ER介导,并可能与细胞内钙动员有关。

17#beta#-estradiol induced nitric oxide release in vascular endothelial cells

Wang Tinghuai, Yang Dan, Liu Peiqing, Gong Suzhen, Lu Wei, Pan Jingyun

Department of Physiology,Sun Yat-sen University of Medical Sciences.Guangzhou 510089,Guangdong

Abstract

Bovine aortic endothelial cells (BAECs) were used to study the effect of 17#beta#-estradiol (E_(2)) on nitric oxide (NO) release, nitric oxide synthase (eNOS) mRNA expression and intracellular free calcium concentration ([Ca~(2+)]_(i)) and modulation of the effect of E_(2) by estrogen receptor (ER) antagonist tamoxifen and NOS inhibitor L-NAME. E_(2) (10~(-12)-10~(-8) mol/L) induced NO release of BAECs in a concentration-dependent manner and the abundant expression of eNOS mRNA in BAECs increased obviously after treatment with E_(2) (10~(-8) mol/L) for 48 h.

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引用本文：

王庭槐, 杨丹, 刘培庆, 龚素珍, 鲁伟, 潘敬运. 17#beta#-雌二醇诱导血管内皮细胞一氧化氮释放及其与细胞内钙的关系[J]. 生理学报 2000; 52 (6): .

Wang Tinghuai, Yang Dan, Liu Peiqing, Gong Suzhen, Lu Wei, Pan Jingyun. 17#beta#-estradiol induced nitric oxide release in vascular endothelial cells. Acta Physiol Sin 2000; 52 (6): (in Chinese with English abstract).