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兔膈肌骨骼肌型DRPR_(#alpha#1)亚单位和RyRs的mRNA与蛋白表达测定

张睢扬, 刘刚, 王东林, 郭先健, 钱桂生

第三军医大学新桥医院全军呼吸内科研究所.重庆 400037

摘要

兔膈肌可见较高水平的骨骼肌型_(#alpha#1)-亚单位和RyR_(2) mRNA与蛋白表达;较低水平的RyR_(3) mRNA与蛋白表达。表明兔膈肌钙释放单位由骨骼肌型DHPR、RyR_(1)和RyR_(3)构成,Ca~(2+)释放可能具有变构耦联和CICR耦联两种形式,RyR_(3)的存在可能是膈肌ECC依赖于细胞外Ca~(2+)的主要原因。

mRNA and protein expression of skeletal DHPR_(#alpha#1) and RyRs in diaphragm muscle of rabbits

Zhang Suiyang, Liu Gang, Wang Donglin, Guo Xianjian, Qian Guisheng

Institute of Respiratory Diseases of the New Bridge Hospital,The Third Military Medical University.Chongqing 400037

Abstract

To detect mRNA and protein expression of skeletal dihydropridine receptor isoform alpha_(1) subunit and ryanodine receptor 1 and 3 in diaphragm muscle of rabbits, the coupling mode and characteristics of Ca~(2+) release were explored. Reverse transcription PCR, in situ hybridization and immunohistochemical methods were employed. A higher level of mRNA and protein expression of DHPR_(#alpha#1) and RyR_(1), and a lower level of mRNA expression of RyR_(3) were found. It is suggested that the calcium release unit may consist of skeletal DHPR isoform, RyR_(1) and RyR_(3), and there may be two kinds of Ca~(2+) release mode via conformational changes in linked proteins and Ca~(2+)-induced Ca~(2+) release (CICR) in diaphragm muscle of rabbits.

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引用本文：

张睢扬, 刘刚, 王东林, 郭先健, 钱桂生. 兔膈肌骨骼肌型DRPR_(#alpha#1)亚单位和RyRs的mRNA与蛋白表达测定[J]. 生理学报 2000; 52 (6): .

Zhang Suiyang, Liu Gang, Wang Donglin, Guo Xianjian, Qian Guisheng. mRNA and protein expression of skeletal DHPR_(#alpha#1) and RyRs in diaphragm muscle of rabbits. Acta Physiol Sin 2000; 52 (6): (in Chinese with English abstract).