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烫伤对大鼠下丘脑视上核内皮素-1基因转录和蛋白含量的影响

蒋应明, 袁文俊, 向正华, 缪为民, 林丽, 李玲, 焦炳华

第二军医大学生理学教研室.上海 200433

摘要

用原位杂交和免疫组织化学方法观察了烫伤后下丘脑视上核(SON)内皮素-1(ET-1)基因转录和蛋白含量的变化,并用通用图像颗粒分析法估计ET-1 mRNA阳性杂交信号的强度和ET-1样免疫反应物(ET-1-ir)的免疫反应强度。结果表明,烫伤后大鼠下丘脑SON ET-1 mRNA含量增加,而ET-1-ir含量在烫伤后180min内维持在低水平。

Effect of scald on gene transcription and content of endothelin-1 in supraoptic nucleus of rat hypothalamus

Jiang Yingming, Yuan Wenjun, Xiang Zhenghua, Miao Weimin, Lin Li, Li Ling, Jiao Binghua

Department of Physiology,Second Military Medical University.Shanghai 200433

Abstract

Endothelin-1 (ET-1) gene transcription and endothelin-1-immunoreactivity (ET-1-ir) in the supraoptic nucleus (SON) of rat hypothalamus were respectively observed by in situ hybridization and immunohistochemistry after scald. Intensity of ET-1 mRNA and endothelin-1-immunoreactivity (ET-1-ir) was quantified by image analysis.

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引用本文：

蒋应明, 袁文俊, 向正华, 缪为民, 林丽, 李玲, 焦炳华. 烫伤对大鼠下丘脑视上核内皮素-1基因转录和蛋白含量的影响[J]. 生理学报 2000; 52 (5): 385-389.

Jiang Yingming, Yuan Wenjun, Xiang Zhenghua, Miao Weimin, Lin Li, Li Ling, Jiao Binghua. Effect of scald on gene transcription and content of endothelin-1 in supraoptic nucleus of rat hypothalamus. Acta Physiol Sin 2000; 52 (5): 385-389 (in Chinese with English abstract).