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红细胞生成素3^端增强子在肺动脉平滑肌细胞低氧性增殖调控中的作用

叶红, 郝天玲, 金咸rong

同济医科大学病理生理学教研室.湖北,武汉 430030

摘要

观察红细胞生成素(EPO)3^端增强子片段对培养的猪肺动脉平滑肌细胞(PASMCs)的内皮依赖性和非内皮依赖性低氧性增殖的影响。结果为:(1)低氧24h后PASMCs明显增殖,转入野生型EPO3^端增强子片段可被抑制,而转入突变型片段无此作用;(2)肺动脉内皮细胞(PAECs)低氧24h,其条件培养液有明显的促PASMCs增殖作用,将野生型EPO3^端增强子片段先转入PAECs,此作用明显减弱,而转入突变型片段则无明显影响。

Effect of erythropoietin 3^-enhancer on proliferation of pulmonary artery smooth muscle cells induced by hypoxia

Ye Hong, Hao Tianling, Jin Xianrong

Department of Pathophysiology,Tongji Medical University.Wuhan 430030,Hubei

Abstract

The effects of erythropoietin (EPO) 3^-enhancer on endothelium-dependent and endothelium-independent proliferation caused by hypoxia in cultured porcine pulmonary artery smooth muscle cells (PASMCs) were investigated with MTT test, H~(3)-TdR incorporation and flow-cytometry.

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引用本文：

叶红, 郝天玲, 金咸rong. 红细胞生成素3^端增强子在肺动脉平滑肌细胞低氧性增殖调控中的作用[J]. 生理学报 2000; 52 (5): 355-359.

Ye Hong, Hao Tianling, Jin Xianrong. Effect of erythropoietin 3^-enhancer on proliferation of pulmonary artery smooth muscle cells induced by hypoxia. Acta Physiol Sin 2000; 52 (5): 355-359 (in Chinese with English abstract).