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用RDA技术寻找肝再生相关基因的初步研究

许望翔, 汪思应, 魏汉东, 杨晓明

军事医学科学院放射医学研究所.北京 100850

摘要

利用表达性差异显示分析(RDA)技术,研究大鼠2/3肝部分切除后1h肝组织中基因的选择性表达,建立了大鼠2/3肝部分切除术后1h再生肝组织选择性表达基因EST库。该库约含3×10~(4)个独立克隆,其中95%以上的克隆含有插入片段,长度约200-700bp不等,对随机挑出的52个克隆的序列分析表明其中大多数基因与肝再生调控相关(38/52)。10株未报道序列经RNA杂交证实,其中6株与肝再生相关。

Isolation of regulation genes related with liver regeneration by representational difference analysis

Xu Wangxiang, Wang Siying, Wei Handong, Yang Xiaoming

Institute of Radiation Medicine,Academy of Military Medical Sciences.Beijing 100850

Abstract

mRNA isolated from 2/3 partially hepatectomied rats was used as a "tester" for representational difference analysis (RDA). The subtracted tester cDNA was cloned into a T-vector and a rat regeneration liver-specific EST pool was constructed, which contained about 3×10~(4) independent clones. A sequence analysis of 52 clones randomly picked up from this pool indicated that the liver regeneration specific sequences were enriched, and the results of RNA blots revealed some novel genes in association with liver regeneration.

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引用本文：

许望翔, 汪思应, 魏汉东, 杨晓明. 用RDA技术寻找肝再生相关基因的初步研究[J]. 生理学报 2000; 52 (4): .

Xu Wangxiang, Wang Siying, Wei Handong, Yang Xiaoming. Isolation of regulation genes related with liver regeneration by representational difference analysis. Acta Physiol Sin 2000; 52 (4): (in Chinese with English abstract).