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胃动素和胃泌素引起大鼠胃窦平滑肌细胞收缩的信号转导通路

王玲, 周吕

医科院基础医学研究所、中国协和医科大学基础医学院生理研究室.北京 100005

摘要

应用大鼠游离胃窦平滑肌细胞,观察胃动素和胃泌素对胃窦平滑肌细胞收缩作用的胞内信号转导通路。结果显示:(1)胃动素和胃泌素对胃窦平滑肌细胞均有收缩作用;(2)G_(ai-3)抗体可抑制胃动素和胃泌素加强胃窦平滑肌细胞的收缩,胃动素、胃泌素明显增加G_(ai-3)抗体与[~(35)S]GTP#gamma#S的结合;(3)磷脂酶C抑制剂U-73122、三磷酸肌醇受体拮抗剂肝素可抑制胃动素和胃泌素引起的胃窦平滑肌细胞的收缩。

Signal transduction pathway for antral smooth muscle cell contraction induced by motilin and gastrin in rats

Wang Ling, Zhou Lu

Department of Physiology,Institute of Basic Medical Sciences,Chinese Academy of Medical Sciences and Peking Union Medical College.Beijing 100005

Abstract

Signal transduction pathway that mediates motilin- and gastrin-induced contraction of antral smooth muscle cells was investigated in rats. The results are as follows. (1) Motilin and gastrin induced contraction of isolated gastric smooth muscle cells in a dose-dependent manner. (2) Motilin- and gastrin-induced antral smooth muscle cell contraction was inhibited by antibodies against G_(ai-3). Motilin and gastrin caused an increase of Guanosine-5^-(3-[~(35)S]thio) triphosphate ([~(35)S]GTP#gamma#S) binding of G_(ai-3). (3) Contraction of antral muscle cells induced by motilin and gastrin was inhibited by the phospholipase C (PLC) inhibitor U-73122 and the IP_(3) receptor antagonist heparin.

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引用本文：

王玲, 周吕. 胃动素和胃泌素引起大鼠胃窦平滑肌细胞收缩的信号转导通路[J]. 生理学报 2000; 52 (4): .

Wang Ling, Zhou Lu. Signal transduction pathway for antral smooth muscle cell contraction induced by motilin and gastrin in rats. Acta Physiol Sin 2000; 52 (4): (in Chinese with English abstract).