过刊浏览

周围神经损伤后外源性GDNF对神经元的保护作用

陈哲宇, 曹莉, 路长林, 何成, 鲍xuan

第二军医大学神经生物学教研室.上海 200433；中科院上海脑研究所.上海 200031

摘要

采用硅管套接大鼠切断的坐骨神经模型,局部给予胶质细胞源性神经营养因子(GDNF),应用尼氏染色、酶组织化学染色方法,观察到外源性GDNF能减少脊髓修复侧前角运动神经元死亡的数目,降低脊髓前角运动神经元及脊神经节感觉神经元中胆碱酯酶及酸性磷酸酶变化的幅度。

Protective effect of exogenous glial cell line-derived neurotrophic factor on neurons after aciatic nerve injury in rats

Chen Zheyu, Cao Li, Lu Changlin, He Cheng, Bao Xuan

Department of Neurobiology,Second Military Medical University.Shanghai 200433；China

Abstract

The aim of the study was to investigate the effect of exogenous glial cell line-derived neurotrophic factor (GDNF) on spinal cord neurons after sciatic nerve axotomy. Upon silicone-tubulization of transected sciatic nerve in the adult rat, either 0.9% saline or GDNF solution was injected into the silicone chamber. It was observed by Nissl and enzyme histochemistry staining that exogenous GDNF decreased lesion-induced motor neuron death in lateral nucleus of spinal anterior horn and the changes in activity of cholinesterase and acid phosphatase in spinal cord and sensory ganglions.

收稿日期：　　录用日期：

通讯作者：　　E-mail:

引用本文：

陈哲宇, 曹莉, 路长林, 何成, 鲍xuan. 周围神经损伤后外源性GDNF对神经元的保护作用[J]. 生理学报 2000; 52 (4): .

Chen Zheyu, Cao Li, Lu Changlin, He Cheng, Bao Xuan. Protective effect of exogenous glial cell line-derived neurotrophic factor on neurons after aciatic nerve injury in rats. Acta Physiol Sin 2000; 52 (4): (in Chinese with English abstract).