过刊浏览

质粒DNA增强大鼠缺血骨骼肌肌浆网钙转运功能

姜志胜, 赵文, 杨永宗, 唐朝枢, 汤健, 贾弘ti, 刘乃奎

衡阳医学院心血管病研究所.湖南,衡阳 421001；北京医科大学心血管基础研究所.北京 100083；北京医科大学生物化学与分子生物学系.北京 100083

摘要

在大鼠肢体缺血模型上观察了质粒pcDNA3对缺血骨骼肌肌浆网(SR)Ca~(2+)转运的影响。结果显示,骨骼肌缺血时SR Ca~(2+)转运(Ca~(2+)摄入与释放)较非缺血肌肉增强,而质粒pcDNA3与SR上DNA结合蛋白结合之后,可进一步增强缺血骨骼肌SR Ca~(2+)摄入(P<0.01)及释放速率(P<0.05)。提示质粒DNA对正常及缺血大鼠骨骼肌的SR Ca~(2+)转运能力均有影响,其临床病理生理意义值得进一步研究。

Plasmid pcDNA3 enhances Ca~(2+) transport of sarcoplasmic reticulum in ischemic skeletal muscle of rats

Jiang Zhisheng, Zhao Wen, Yang Yongzong, Tang Chaoshu, Tang Jian, Jia Hongti, Liu Naikui

Research Institute of Cardiovascular Diseases,Hengyang Medical College.Hengyang 421001；Institute of Cardiovascular Research,Beijing Medical University.Beijing 100083；China

Abstract

The effects of plasmid pcDNA3 on Ca~(2+) transport of sarcoplasmic reticulum (SR) in ischemic skeletal muscle was investigated. The results show that Ca~(2+) transport (including Ca~(2+) uptake and Ca~(2+) release) rate of SR in ischemic skeletal muscle was markedly increased compared with that in non-ischemic muscle (P<0.01 or P<0.05). After plasmid pcDNA3 bound to the DNA binding proteins of SR, Ca~(2+) transport of SR was further increased. The results suggest that the effect of plasmid DNA on the ability of Ca~(2+) transport in SR of ischemic skeletal muscle is the same as is observed in normal skeletal muscle. The pathophysiological significance of the present finding deserves further exploration.

收稿日期：　　录用日期：

通讯作者：　　E-mail:

引用本文：

姜志胜, 赵文, 杨永宗, 唐朝枢, 汤健, 贾弘ti, 刘乃奎. 质粒DNA增强大鼠缺血骨骼肌肌浆网钙转运功能[J]. 生理学报 2000; 52 (3): .

Jiang Zhisheng, Zhao Wen, Yang Yongzong, Tang Chaoshu, Tang Jian, Jia Hongti, Liu Naikui. Plasmid pcDNA3 enhances Ca~(2+) transport of sarcoplasmic reticulum in ischemic skeletal muscle of rats. Acta Physiol Sin 2000; 52 (3): (in Chinese with English abstract).