过刊浏览

水稻乙醛脱氢酶基因的克隆及其在不育系中的表达

徐秉芳, 邢彦彦, 王宗阳, 张景六, 朱珊珊, 洪孟民

中科院上海植物生理研究所.上海 200032

摘要

从水稻中分离了乙醛脱氢酶基因(Osaldh)的全长cDNA,序列分析显示Osaldh cDNA含有一个完整的编码549个氨基酸的开放阅读框,其编码蛋白OSALDH的N端为预期的线粒体前导肽,中部具有醛类脱氢酶基因家族的酶催化活性中心,OSALDH与玉米、烟草、人类的线粒体乙醛脱氢酶同源性分别高达87%、77%、59%。Northern分析显示,幼根中Osaldh的表达水平高于幼苗、幼穗,不育品种幼穗中Osaldh的转录水平普遍高于对应的可育恢复系。植物线粒体乙醛脱氢酶的功能及其与雄性不育的关系值得进一步研究。

关键词： 乙醛脱氢酶基因; 水稻; 雄性不育

Cloning, characterization of cDNA encoding rice aldehyde dehydrogenase, and analysis of its expression in male-sterile lines

Xu Bingfang, Xing Yanyan, Wang Zongyang, Zhang Jingliu, Zhu Shanshan, Hong Mengmin

Shanghai Institute of Plant Physiology,Chinese Academy of Sciences.Shanghai 200032

Abstract

We also compared the expression of Osaldh between the male-sterile and fertile varieties, the result showed that the transcript level of male-sterile lines was generally higher. The relationship between the expression level of Osaldh and male-sterility was discussed.

Key words: Aldehyde dehydrogenase gene;Oryza sativa;Male sterility

收稿日期：　　录用日期：

通讯作者：　　E-mail:

引用本文：

徐秉芳, 邢彦彦, 王宗阳, 张景六, 朱珊珊, 洪孟民. 水稻乙醛脱氢酶基因的克隆及其在不育系中的表达[J]. 生理学报 2000; 52 (3): .

Xu Bingfang, Xing Yanyan, Wang Zongyang, Zhang Jingliu, Zhu Shanshan, Hong Mengmin. Cloning, characterization of cDNA encoding rice aldehyde dehydrogenase, and analysis of its expression in male-sterile lines. Acta Physiol Sin 2000; 52 (3): (in Chinese with English abstract).