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ABA诱导蚕豆气孔保卫细胞H_(2)O_(2)的产生

苗雨晨, 宋纯鹏, 董发才, 王学臣

河南大学生物学系.河南,开封 475001；中国农业大学植物生理生化开放实验室.北京 100094

摘要

以蚕豆叶片下表皮为材料,将荧光探针HPTS导入蚕豆气孔保卫细胞内,利用荧光光谱和激光共聚集显微技术,检测了ABA诱导蚕豆气孔关闭过程中H_(2)O_(2)的产生。结果表明,ABA和100#mu#mol/L的H_(2)O_(2)可以迅速猝灭HPTS的荧光,CAT几乎可完全阻止9#mu#l 0.2mmol/L的ABA引起荧光猝灭。因此,ABA可以诱导气孔保卫细胞产生H_(2)O_(2),其产生的强度和速度与ABA的浓度直接有关,并且推测:这种H_(2)O_(2)产生可能是ABA诱导气孔关闭过程中信号转导链的一个中间成分。

关键词： 蚕豆; 保卫细胞; ABA; H_(2)O_(2); 信号转导

ABA-induced hydrogen peroxide generation in guard cells of vicia faba

Miao Yuchen, Song Chunpeng, Dong Facai, Wang Xuechen

Department of Biology,Henan University.Kaifeng 475001,Henan；China

Abstract

The results obtained by laser scanning confocal microscopy suggested that addition of exogenous aBA resulted in a rapid decrease in fluorescence in most cellular compartments of the guard cells. Above data support the hypothesis that H_(2)O_(2) is involved in the signal transduction pathway of ABA-induced stomatal closure.

Key words: Vicia faba;Guard cells;Abscisic acid;Hydrogen peroxide;Signal transduction

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引用本文：

苗雨晨, 宋纯鹏, 董发才, 王学臣. ABA诱导蚕豆气孔保卫细胞H_(2)O_(2)的产生[J]. 生理学报 2000; 52 (1): .

Miao Yuchen, Song Chunpeng, Dong Facai, Wang Xuechen. ABA-induced hydrogen peroxide generation in guard cells of vicia faba. Acta Physiol Sin 2000; 52 (1): (in Chinese with English abstract).