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活性氧对苋菜磷酸烯醇式丙酮酸羧化酶活性的影响

林植芳, 彭长连, 林桂珠

中科院华南植物研究所.广东,广州 510650

摘要

用活性氧H_(2)O_(2)、O~(.)_(2)和OH·处理苋菜部分纯化的PEP羧化酶(PEPC)15min后,活性降低10%-17%,抑制程度与活性氧种类及其浓度有关。OH·的清除剂DMSO可提高PEPC活性29%-48%,并相应影响紫外差光谱峰。结果表明氧化胁迫导致PEPC构象变化,酶蛋白氧化断裂或降解,活性下降。

关键词： 活性氧; PEP羧化酶活性; 苋菜; 紫外差光谱

Effect of active oxygen on activity of phosphoenolpyruvate carboxylase from Amaranthus tricolor

Lin Zhifang, Peng Changlian, Lin Guizhu

South China Institute of Botany,Chinese Academy of Sciences.Guangzhou 510650,Guangdong

Abstract

Phosphoenolpyruvate carboxylase (PEPC) was partially purified from leaves of Amaranthus tricolor, then treated with active oxygen species such as H_(2)O_(2), O~(.)_(2) and OH· for 15 min. Under oxidative condition, PEPC activity was inhibited by 10%-17% depending on the species and concentration of active oxygen. DMSO, a hydroxyl radical scavenger, stimulated the PEPC activity by 29%-48%, and altered the spectra of ultraviolet bsorption and differential absorption spectra. These results imply that oxidative stress leads to the modification of structure and conformation of PEPC and a decrease in activity.

Key words: Active oxygen;Activity of phosphoenolpyruvate carboxylase;Amaranthus tricolor;Ultraviolet difference spectra

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引用本文：

林植芳, 彭长连, 林桂珠. 活性氧对苋菜磷酸烯醇式丙酮酸羧化酶活性的影响[J]. 生理学报 2000; 52 (1): .

Lin Zhifang, Peng Changlian, Lin Guizhu. Effect of active oxygen on activity of phosphoenolpyruvate carboxylase from Amaranthus tricolor. Acta Physiol Sin 2000; 52 (1): (in Chinese with English abstract).