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烟草细胞乙醇分解代谢能力的离体调控

郑迎冬, 杨跃生, 陈玉芬, 杨军

华南农业大学生物技术学院.广东,广州 510642

摘要

报告了一些重要因子对培养在含有乙醇的培养基上的烟草细胞的乙醇分解代谢能力(ECA)的调控效果。在供试的5种生长素和2种细胞分裂素中,IBA 4mg/L和Kt 0.05mg/L最有利于促进ECA,而NAA 2mg/L和Kt 0.025mg/L则最有利于支持处在乙醇胁迫下的细胞的增殖。实验中还发现提高维生素浓度有利于细胞的ECA。通过将细胞从低乙醇浓度的培养基转移到较高乙醇浓度的培养基中,能够诱导细胞耐受更高浓度的乙醇并增强细胞的ECA。研究结果可以应用于制备适合分离乙醇分解代谢途径中的酶的烟草细胞材料。

关键词： 乙醇代谢; 离体培养; 烟草; 植物激素

In vitro regulation of the ethanol catabolism ability of tobacco (Nicotiana tabacum L.) cells

Zheng Yingdong, Yang Yuesheng, Chen Yufen, Yang Jun

College of Biotechnology,South China Agricultural University.Guangzhou 510642,Guangdong

Abstract

The regulatory effects of some key factors on the ethanol catabolism ability (ECA) of tobacco cells were investigated using cells cultured on medium supplemented with ethanol. Among the five kinds of auxin analogues and two kinds of cytokinins tested, IBA 4 mg/L with kinetin 0.05 mg/L was the most effective hormone combination for stimulating the ECA, while NAA 2 mg/L within kinetin 0.25 mg/L was the most effective one for supporting the cell growth under ethanol stress. Raising the concentrations of vitamins in the media was found to be conducive to the ECA of the cells. By transferring cells consequently to media with progressively increasing levels of ethanol concentration, the cells could be induced to stand higher concentrations of ethanol with an enhanced ECA. The results obtained in the present experiments can be applied to the preparation of proper tobacco cells materials for the isolation of enzymes in the ethanol catabolism pathway.

Key words: Ethanol metabolism;In vitro culture;Nicotiana tabacum;Plant hormone

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引用本文：

郑迎冬, 杨跃生, 陈玉芬, 杨军. 烟草细胞乙醇分解代谢能力的离体调控[J]. 生理学报 2000; 52 (1): .

Zheng Yingdong, Yang Yuesheng, Chen Yufen, Yang Jun. In vitro regulation of the ethanol catabolism ability of tobacco (Nicotiana tabacum L.) cells. Acta Physiol Sin 2000; 52 (1): (in Chinese with English abstract).