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新生大鼠心室成纤维细胞条件培养液的促心肌细胞肥大作用

龚素珍, 刘培庆, 鲁伟, 符史干, 潘敬运

中山医科大学生理教研室.广东,广州 510089

摘要

利用新生大鼠心室成纤维细胞条件培养液孵育心肌细胞,证实成纤维细胞条件培养液能明显增大心肌细胞的表面积, 增加心肌细胞的蛋白含量和[~(3)H]-亮氨酸([~(3)H]-Leu)的掺入,上述作用以d3的条件培养液作用最强,具有剂量依赖性. ET_(A)受体拮抗剂BQ_(123)能部分阻断成纤维细胞条件培养液促进心肌细胞肥大的作用,而AT_(1)受体拮抗剂CV11974和#alpha#-肾上腺素受体拮抗剂regitin却无此效果。

关键词： 成纤维细胞; 心肌细胞; 肥大; 细胞培养; 心脏

Cardiocyte hypertrophy induced by cultured neonatal rat ventricular fibroblast conditioned growth medium

Gong Suzhen, Liu Peiqing, Lu Wei, Fu Shigan, Pan Jingyun

Department of Physiology,Sun Yat-sen University of Medical Sciences.Guangzhou 510089,Guangdong

Abstract

The present work demonstrated that cultured neonatal rat ventricular fibroblast conditioned growth medium (FCGM) could significantly increase cell surface area and protein content and promote [~(3)H]-Leucine incorporation on neonatal rat cardiomyocyte. The above effect was strongest on the third day, and was dose-dependent. BQ_(123), on ET-A receptor antagonist, significantly blocked the effect, while CV11974, an AngII I-type receptor antagonist, and regitin, an #alpha#-adrenergic receptor antagonist, did not.

Key words: Fibroblast;Myocyte hypertrophy;Cell;Culture;Heart

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引用本文：

龚素珍, 刘培庆, 鲁伟, 符史干, 潘敬运. 新生大鼠心室成纤维细胞条件培养液的促心肌细胞肥大作用[J]. 生理学报 2000; 52 (1): .

Gong Suzhen, Liu Peiqing, Lu Wei, Fu Shigan, Pan Jingyun. Cardiocyte hypertrophy induced by cultured neonatal rat ventricular fibroblast conditioned growth medium. Acta Physiol Sin 2000; 52 (1): (in Chinese with English abstract).