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水稻蜡质基因第一内含子碱基突变引起其RNA二级结构的可能变化

蔡秀玲, 王宗阳, 邢彦彦, 张景六, 洪孟民

中科院上海植物生理研究所.上海 200032

摘要

通过体外转录得到籼稻品种232蜡质基因第一内含子5^端430bp的ssRNA分子,以及在此区域发生了自然突变的粳稻品种寒丰蜡质基因第一内含子5^端同样长度的ssRNA分子。部分变性胶电泳结果表明两种ssRNA分子的迁移速率不同。将两种ssRNA分子的核酸序列用计算机分析,表明此两种ssRNA分子能形成不同的茎-环结构,自由能值也有差异。对突变引起的这些不同与两种水稻品种蜡质基因转录本剪接的差异进行了讨论。

关键词： 二级结构; 第一内含子; 蜡质基因; 水稻

Alteration of RNA secondary structure of rice waxy intron 1 caused by naturally ocurred mutations

Cai Xiuling, Wang Zongyang, Xing Yanyan, Zhang Jingliu, Hong Mengmin

Shanghai Institute of Plant Physiology,Chinese Academy of Sciences.Shanghai 200032

Abstract

Two 430 nucleotides ssRNAs were produced in vitro from two 430 bp DNA fragments containing the 5^ part of intron 1 of rice cultivars 232 and Hanfeng, respectively. Nucleotide sequences of the Wx intron 1 in cultivar 232 (high amylose content) and Hanfeng (intermediate amylose content) differ by sixteen individual bases. While these two ssRNA molecules were resolved by electrophoresis in partially-denatured polyacrylamide gel, showing that they migrated differently. Computer analysis of the secondary structure of RNA showed that the 5^ region of the Wx RNA sequence in intron 1 of cultivar 232 could form a stem-loop structure, which differs substantially from that of cultivar Hanfeng. The free energy values of the most stable structures of these two ssRNAs are different, too. Differences in RNA structure and splicing patterns of Wx transcript caused by the sixteen individual bases mutations are discussed.

Key words: Decondary structure;Intron 1;Wx gene;Rice

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引用本文：

蔡秀玲, 王宗阳, 邢彦彦, 张景六, 洪孟民. 水稻蜡质基因第一内含子碱基突变引起其RNA二级结构的可能变化[J]. 生理学报 2000; 52 (1): .

Cai Xiuling, Wang Zongyang, Xing Yanyan, Zhang Jingliu, Hong Mengmin. Alteration of RNA secondary structure of rice waxy intron 1 caused by naturally ocurred mutations. Acta Physiol Sin 2000; 52 (1): (in Chinese with English abstract).