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应急大鼠肾上腺髓质嗜铬细胞颗粒数目与其钙含量的关系

刘懿, 凌诒萍, 俞彰, 吴正泉, 钟慈声

上海医科大学生物物理学教研室.上海 200032

摘要

采用电镜细胞立体形态计量法及电镜X射线显微定量分析术,对制动应急大鼠的肾上腺髓质细胞内嗜铬颗粒数密度和颗粒内Ca浓度变化进行测量。结果显示,两者在制动过程中均呈进行性下降,但颗粒内钙浓度的下降快于颗粒数目的减少(P<0.01)。该结果支持颗粒内钙释放入胞质,参与胞质内游离钙浓度的升高,进而激发颗粒胞吐的假设,为证实嗜铬颗粒是一种细胞内钙库并参与细胞分泌提供了实验依据。

关键词： 肾上腺髓质细胞; 嗜铬颗粒; 颗粒数密度; 钙含量; 电镜X射线显微定量分析

The relationship between the chromaffin granule number and the granule calcium content in the adrenal medulla of restrained rats

Liu Yi, Ling Yiping, Yu Zhang, Wu Zhengquan, Zhong Cisheng

Department of Biophysics,Shanghai Medical University.Shanghai 200032

Abstract

Morphometry of numerical density and electron probe X-ray quantitative microanalysis were used to measure the alterations in the number and calcium content of chromaffin granules in adrenal medulla cells during emergency reaction of restrained rats. It was found that both the alterations showed monotonic decrease during the period of restraint, but the rate of decrease of the calcium content was significantly faster than that of the granules. These data are consistent with the hypothesis that calcium release from the granules may increase the cytoplasmic calcium concentration, which again triggers off the granule exocytosis.

Key words: adult rat testicular;Denervations;Unilateral;Castrations testosterone gonadotropin;

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引用本文：

刘懿, 凌诒萍, 俞彰, 吴正泉, 钟慈声. 应急大鼠肾上腺髓质嗜铬细胞颗粒数目与其钙含量的关系[J]. 生理学报 2000; 52 (1): .

Liu Yi, Ling Yiping, Yu Zhang, Wu Zhengquan, Zhong Cisheng. The relationship between the chromaffin granule number and the granule calcium content in the adrenal medulla of restrained rats. Acta Physiol Sin 2000; 52 (1): (in Chinese with English abstract).