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c-erbB_(2)对大鼠黄体细胞hCG诱导的孕酮分泌的影响

金萱, 方廉, 郑月慧, 钟志胜, 况海斌

江西医学院生理教研室.江西, 南昌 330006

摘要

反义c-erbB_(2)以剂量相关方式抑制黄体细胞hCG诱导的孕酮的产生,同时使c-erbB_(2)蛋白染色阳性的黄体细胞百分数下降,无义tat ODN没有相应的作用。10~(-4) mol/L的二丁酰cAMP能明显反转反义c-erbB_(2) ODN对孕酮产生和c-erbB_(2)表达的抑制作用,钙离子通道阻断剂维拉帕米和蛋白抑制剂CYX对此抑制作用有协同效应。

关键词： 黄体细胞; c-erbB_(2); 反义寡脱氧核苷酸; 孕酮

Effects of c-erbB_(2) on the hCG-induced progesterone production of rat luteal cells

Jin Xuan, Wang Lian, Zheng Yuehui, Zhong Zhisheng, Kuang Haibin

Department of Physiology,Jiangxi Medical College.Nanchang 330006,Jiangxi

Abstract

Antisense c-erbB_(2) ODN inhibited hCG-induced progesterone production in a dose-dependent manner; and the percentage of luteal cells stained for c-erbB_(2) protein was decreased. But nonsense tat ODN had no similar effect. The effect of dbcAMP was in contrast with that of antisense c-erbB_(2) ODN, which suppressed the progesterone production and reduced the percentage of luteal cells stained for c-erbB_(2) protein. On the other hand, both verapamil and CYX enhanced the inhibition of antisense c-erbB_(2) ODN. The result of the present investigation suggests that cAMP, Ca~(2+)-dependent pathway participates in the expression of protooncogene.

Key words: Luteal cells;c-erbB_(2);Antisense oligodeoxynucletoide;Progesterone

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引用本文：

金萱, 方廉, 郑月慧, 钟志胜, 况海斌. c-erbB_(2)对大鼠黄体细胞hCG诱导的孕酮分泌的影响[J]. 生理学报 2000; 52 (1): .

Jin Xuan, Wang Lian, Zheng Yuehui, Zhong Zhisheng, Kuang Haibin. Effects of c-erbB_(2) on the hCG-induced progesterone production of rat luteal cells. Acta Physiol Sin 2000; 52 (1): (in Chinese with English abstract).