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小鼠骨髓内皮细胞分泌的细胞因子对造血干/祖细胞增殖的影响

李卫民, 黄艳红, 蒋德昭, 王绮如

湖南医科大学血液生理研究室.湖南,长沙 410078

摘要

应用小鼠骨髓内皮细胞株细胞传代培养,收集无血清条件培养液(mBMEC-CM),经超滤分成大于10kD和小于10kD两组分,分别观察两组分的mBMEC-CM对小鼠骨髓造血干/祖细胞CFU-GM,HPP-CFC,CFU-E,BFU-E和CFU-Meg的影响。

关键词： 骨髓内皮细胞; 无血清条件培养液; 细胞因子; 造血调控

The effects of cytokines produced by bone marrow endothelial cells on the regulation of hematopoiesis

Li Weimin, Huang Yanhong, Jiang Dezhao, Wang Qiru

Research Laboratory of Blood Physiology,Hunan Medical University.Changsha 410078,Hunan

Abstract

In this study, the effects of bone marrow endothelial cell conditioned medium (BMEC-CM) on hematopoietic progenitors and the expression of cytokines mRNA of BMEC were investigated. BMEC-CM was ultrafiltrated in Centriprep-10, an ultrafiltering concentrator. The retentate (MW>10 kD) and filtrate (MW<10 kD) were obtained. The effects of different components of BMEC-CM on the murine hematopoietic progenitors were examined.

Key words: Bone marrow endothelial cells;Serum free condition;Medium cytokine;Regulation of hematopoiesis

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引用本文：

李卫民, 黄艳红, 蒋德昭, 王绮如. 小鼠骨髓内皮细胞分泌的细胞因子对造血干/祖细胞增殖的影响[J]. 生理学报 2000; 52 (1): .

Li Weimin, Huang Yanhong, Jiang Dezhao, Wang Qiru. The effects of cytokines produced by bone marrow endothelial cells on the regulation of hematopoiesis. Acta Physiol Sin 2000; 52 (1): (in Chinese with English abstract).