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#beta#-半乳糖苷酶基因在猕猴桃果实成熟过程的表达

陈昆松, 张上隆, Ross G S

浙江大学生命科学学院植物分子生理学实验室.浙江,杭州 310029；浙江大学园艺系.浙江,杭州 310029；新西兰国家研究院园艺与食品研究所.奥克兰 92 169

摘要

从成熟中华猕猴桃果实中克隆到了一个#beta#-半乳糖苷酶基因cDNA片段,其长度为747bp,有一由249个氨基酸组成的开放阅读框架,它与苹果、芦笋、绿花椰菜、番茄中#beta#-半乳糖苷酶基因cDNA相应区段的核苷酸同源性为67.3%-70.3%,氨基酸同源性为69.1%-72.7%。用该片段为探针进行Northern分析表明,果实采收时,#beta#-半乳糖苷酶mRNA水平最高,随后呈下降变化,同时#beta#-半乳糖苷酶基因的表达可为外源乙烯所诱导,但在果实乙烯跃变期间#beta#-半乳糖苷酶基因的表达信号无显著变化。文中对#beta#-半乳糖苷酶在猕猴桃果实成熟衰老过程的作用进行了讨论。

关键词： 猕猴桃; 果实; β-半乳糖苷酶基因; 表达模式

Expression pattern of #beta#-galactosidase gene in ripening Actinidia chinensis fruit

Chen Kunsong, Zhang Shanglong, Ross G S

Lab of Plant Molecular Physiology,College of Life Sciences,Zhejiang University.Hangzhou 310029,Zhejiang；China

Abstract

Northern analysis showed that #beta#-galactosidase mRNA was abundant in the fruit at harvest time and then went down. The mRNA level was the lowest at the stage of 24 h ethylene treatment. A transient increase in the mRNA level was seen after 24 h ethylene treatment and no significant change during ethylene climacteric in ripening fruit. The significance of these results is discussed.

Key words: Actinidia chinensis;Fruit;#beta#-galactosidase gene;Expression pattern

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引用本文：

陈昆松, 张上隆, Ross G S. #beta#-半乳糖苷酶基因在猕猴桃果实成熟过程的表达[J]. 生理学报 2000; 52 (2): .

Chen Kunsong, Zhang Shanglong, Ross G S. Expression pattern of #beta#-galactosidase gene in ripening Actinidia chinensis fruit. Acta Physiol Sin 2000; 52 (2): (in Chinese with English abstract).