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甾体激素对神经母细胞株SK-N-SH细胞摄取甘氨酸的快速作用

毕钢, 陈宜张

第二军医大学神经科学研究所.上海 200433

摘要

SK-N-SH细胞高亲和力的甘氨酸摄取是Na~(+)和Cl~(-)依赖性的。皮质酮、孕酮和地塞米松对这种摄取有快速促进作用,雌二醇和脱氧皮质酮无明显作用,表明甾体激素快速作用有特异性。皮质酮作用在10~(-9)-10~(-6)mol/L范围内呈浓度依赖性。皮质酮快速作用不受蛋白质合成抑制剂影响。耦联牛血清白蛋白后,快速作用仍然存在。但细胞外液无Ca~(2+)影响甾体激素快速作用。

关键词： 甾体激素; SK-N-SH细胞; 甘氨酸; 非基因组机制; 摄取

The rapid effects of steroids on glycine uptake in neuroblastoma cell strain SK-N-SH cells

Bi Gang, Chen Yizhang

Neuroscience Research Institute,Second Military Medical University.Shanghai 200433

Abstract

In the present study, glycine uptake in SK-N-SH cells was determined with liquid scintillation technique, and the rapid effects of steroids on glycine uptake in SK-N-SH cells were investigated. The results suggest that the steroid effect on glycine uptake in SK-N-SH cells was nongenomicly mediated.

Key words: Steroids;SK-N-SH cell;Glycine;Nongenomic;Mechanism

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引用本文：

毕钢, 陈宜张. 甾体激素对神经母细胞株SK-N-SH细胞摄取甘氨酸的快速作用[J]. 生理学报 1999; 51 (6): .

Bi Gang, Chen Yizhang. The rapid effects of steroids on glycine uptake in neuroblastoma cell strain SK-N-SH cells. Acta Physiol Sin 1999; 51 (6): (in Chinese with English abstract).