热休克蛋白、蛋白水解酶和磷酸酶在大鼠肝再生中的含量和活性变化
徐存拴, 夏民, 卢爱灵, 李效阳, 李永辉, 赵绪永, 胡轶红
河南师范大学生命科学学院.河南,新乡 453002
摘要
以2/3肝切除大鼠为模型,探讨了PH后酸性和碱性磷酸酶(ACP和AKP)、构成性热休克蛋白70/诱导性热休克蛋白68(HSC70/HSP68)、酸性和中性蛋白水解酶在肝再生期间(0-144h)的动态变化。结果提示,ACT和90kD中性蛋白水解酶可能参与激活G_(0)期肝细胞进入G_(1)期;AKP和HSC70/HSP68可能主要在DNA合成、细胞代谢和增殖方面起作用;27kD酸性蛋白水解酶可能在肝细胞再分化和肝组织重建中有一定作用。
关键词: 肝再生; 构成性热休蛋白70/诱导性热休克蛋白68; 蛋白水解酶; 碱性磷酸酶; 酸性磷酸酶; 酶的原位复性电泳
Changes in the content and activity of HSC70/HSP68, proteinases and phosphatases during liver regeneration
Xu Cunshuan, Xia Min, Lu Ailing, Li Xiaoyang, Li Yonghui, Zhao Xuyong, Hu Yihong
College of Life Science,Henan Normal University.Xinxiang 453002,Henan
Abstract
The ACP and the 90 kD neutral proteinase may participate in activating hepatocytes from G_(0)-phase into G_(1)-phase, and AKP and HSC70/HSP68 may play a role mainly in DNA synthesis, cellular metabolism and proliferation. Furthermore 27 kD acid proteinase may be involved in re-differentiation of hepatocytes and reconstruction of liver tissue.
Key words: Liver regeneration;Constitutive heat shock protein 70/induced heat shock protein 68(HSC70/HSP68);Proteinases acid phosphatase(ACP);Alkaline phosphatase(AKP);Non-denatured electrophoresis;
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引用本文:
徐存拴, 夏民, 卢爱灵, 李效阳, 李永辉, 赵绪永, 胡轶红. 热休克蛋白、蛋白水解酶和磷酸酶在大鼠肝再生中的含量和活性变化[J]. 生理学报 1999; 51 (5): .
Xu Cunshuan, Xia Min, Lu Ailing, Li Xiaoyang, Li Yonghui, Zhao Xuyong, Hu Yihong. Changes in the content and activity of HSC70/HSP68, proteinases and phosphatases during liver regeneration. Acta Physiol Sin 1999; 51 (5): (in Chinese with English abstract).