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睫状神经营养因子对NO引起海马神经元毒性反应的影响

陈秀青, 陈哲宇, 路长林, 何成, 王成海, 鲍xuan

第二军医大学神经科学研究所神经生物学教研室.上海 200433；中科院上海脑研究所.上海 200031

摘要

NO供体硝普钠与S-亚硝基-乙酰青霉胺,NOS底物L-Arg及钙载体ionomycin,均可引起海马神经元存活率下降,LDH漏出增加;提前24h给予不同浓度CNTF,均能提高神经元的存活率,减少LDH漏出,其作用呈剂量依赖性;CNTF可降低ionomycin引起的NO含量升高;CNTF对神经元存活率的影响与NO含量呈显著负相关。

关键词： 睫状神经营养因子; 海马神经元; 一氧化氮; 细胞毒性

Ciliary neurotrophic factor prevents NO mediated cytotoxicity in cultured hippocampal neurons

Chen Xiuqing, Chen Zheyu, Lu Changlin, He Cheng, Wang Chenghai, Bao Xuan

Department of Neurobiology,Institute of Neuroscience Research,Second Military Medical University.Shanghai 200433；China

Abstract

The neurotoxicity was induced by NO donors, such as sodium nitroprusside (SNP), s-nitroso-N-acetylpenicillamine (SNAP), NOS substrate, L-arginine (L-Arg) and ionomycin (a calcium ionophore). Preincubation of the cultures with ciliary neurotrophic factor (CNTF) for 24 h promoted neuronal survival and decreased the LDH efflux and the level of NO induced by ionomycin. The protective effects of CNTF against NO cytotoxicity were concentration-dependent.

Key words: Ciliary neurotrophic factor (CNTF);Hippocampal neurons;Nitric oxide (NO);Cytotoxicity

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引用本文：

陈秀青, 陈哲宇, 路长林, 何成, 王成海, 鲍xuan. 睫状神经营养因子对NO引起海马神经元毒性反应的影响[J]. 生理学报 1999; 51 (5): .

Chen Xiuqing, Chen Zheyu, Lu Changlin, He Cheng, Wang Chenghai, Bao Xuan. Ciliary neurotrophic factor prevents NO mediated cytotoxicity in cultured hippocampal neurons. Acta Physiol Sin 1999; 51 (5): (in Chinese with English abstract).