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根癌农杆菌介导的水稻转化及转基因R_(1)代植株表型特征

章冰, 卫志明

中科院上海植物生理研究所.上海 200032

摘要

用根癌农杆菌介导的方法对2个粳稻品种和4个籼稻品种进行了转化。粳稻成熟胚和籼稻幼胚来源的愈伤组织用携带质粒pGIH的农杆菌EHA101感染,对所有品种均获得较高的愈伤转化频率(20.83%-62.32%)。粳稻“申香粳4号”植株转化频率为17.39%,“秋丰”为9.21%。4个品种籼稻中仅“超丰早1号”获得1株转化植株。Southern杂交分析、GUS染色、T-DNA整合边界序列分析等结果表明外源基因整合入植物基因组中。对R_(1)代分析结果表明外源基因能遗传给后代,大多数株系的分离比符合3:1,也有少数株系分离比异常。对转基因R_(1)代水稻植株表型特征分析结果显示转化植株株高下降,每株分蘖数减少,每株籽粒数明显减少,部分植株播种至开花所需时间延迟。

关键词： 农杆菌; 水稻; 遗传转化; 表型特征

Agrobacterium-mediated transformation of rice and the phenotypic characteristics of the transgenic R_(1) plants

Zhang Bing, Wei Zhiming

Shanghai Institute of Plant Physiology,Chinese Academy of Sciences.Shanghai 200032

Abstract

Genetic transformation mediated at Agrobacterium tumeficiens has been applied to two japonica varieties and four indica varieties of rice (Oryza sativa L.). The frequencies of transgenic calli are high (20.83%-62.32%) for both the japonica and indica varieties tested. The frequency of transgenic plants in japonica variety "Shenxiangjing No. 4" was 17.39%, while that in japonica variety "Qiufeng" was 9.21%. Among the 4 indica varieties, only 1 transgenic plant was obtained in "Chaofengzao No. 1". Integration of foreign genes into the genome of transgenic plants was confirmed by Southern analysis, GUS assay (Plate I-b, e) and boundary sequence analysis of T-DNA in transgenic rice plant.

Key words: Agrobacterium tumeficiens;Oryza sativa L.;Genetic transformation;Phenotypic characteristics

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引用本文：

章冰, 卫志明. 根癌农杆菌介导的水稻转化及转基因R_(1)代植株表型特征[J]. 生理学报 1999; 51 (4): .

Zhang Bing, Wei Zhiming. Agrobacterium-mediated transformation of rice and the phenotypic characteristics of the transgenic R_(1) plants. Acta Physiol Sin 1999; 51 (4): (in Chinese with English abstract).