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多胺对水稻CMS系及其保持系幼穗蛋白质、核酸和活性氧代谢的影响

田长恩, 梁承邺

中科院华南植物研究所.广东,广州 510650

摘要

用D-Arg+MGBG处理保持系,降低花粉可育度,并使其幼穗中蛋白质、DNA和RNA含量以及蛋白酶、RNA酶和DNA酶活性下降,使O~(.)_(2)生成速率和MDA含量上升。Put+Spd+Spm可消除或部分消除D-Arg+MGBG的上述效应(对酶活性的影响除外)。D-Arg+MGBG也使POD、SOD和CAT活性上升,但是,多胺只能降低抑制剂对POD的刺激作用。用Put+Spd+Spm处理不育系,使花粉可育度轻度提高,并使其幼穗蛋白质、DNA和RNA含量略有上升,使蛋白酶、DNA酶和RNA酶活性、O~(.)_(2)生成速率、MDA含量、SOD和CAT活性下降,使POD活性上升。

关键词： 多胺; 蛋白质、DNA、RNA、活性氧代谢; 细胞质雄性不育; 水稻

Effect of polyamine on the metabolism of protein, DNA, RNA and activated oxygen in the panicles of CMS rice and its maintainer line

Tian Chang^en, Liang Chengye

South China Institute of Botany,Chinese Academy of Sciences.Guangzhou 510650,Guangdong

Abstract

In CMS line, treatment with Put+Spd+Spm made pollen fertility, and the contents of protein, DNA and RNA increased to some extent; but made the activities of protease, DNase and RNase decreased. In the maintainer line, treatment with D-Arg+MGBG made pollen fertility and contents of protein, DNA and RNA, and the activities of protease, DNase and RNase decreased. Put+Spd+Spm, however, could partly counteract the effect of D-Arg+MGBG on pollen fertility and contents of protein, DNA and RNA. After being treated with Put+Spd+Spm, the production rate of O~(.)_(2), MDA content and the activities of SOD and CAT in CMS line decreased. At the same time, however, the activity of POD increased. In the maintainer line, the production rate of O~(.)_(2) and MDA content increased by treatment with D-Arg+MGBG. Put+Spd+Spm could counteract this effect. Also, after being treated with D-Arg+MGBG, the activities of POD, SOD and CAT increased. Put+Spd+Spm, nevertheless, could merely decreased the stimulating effect of D-Arg+MGBG on POD. Meanwhile, pollen fertility in the maintainer line was decreased by D-Arg+MGBG.

Key words: Polyamine;Pprotein, DNA, RNA and activated oxygen metabolism;Cytoplasmic male sterility;Rice

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引用本文：

田长恩, 梁承邺. 多胺对水稻CMS系及其保持系幼穗蛋白质、核酸和活性氧代谢的影响[J]. 生理学报 1999; 51 (3): .

Tian Chang^en, Liang Chengye. Effect of polyamine on the metabolism of protein, DNA, RNA and activated oxygen in the panicles of CMS rice and its maintainer line. Acta Physiol Sin 1999; 51 (3): (in Chinese with English abstract).