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NaCl胁迫下小麦突变体和野生型叶片中一些有机溶质累积和基因表达差异

郭房庆, 周建明, 汤章城

中科院上海植物生理研究所.上海 200032

摘要

盐胁迫下突变体和野生型叶片中的脯氨酸累积量均有显著的增加,野生型的增加幅度不仅突变体。至96h,两者含量均下降,但突变体的脯氨酸含量仍高于野生型。100mmol/L的NaCl胁迫72h,突变体叶片中可溶性糖的含量有显著的增加,增加量随盐浓度增加而降低。至96h,各个盐浓度处理的突变体可溶性糖的含量基本恢复到其对照的水平;除100mmol/L盐胁迫处理组外,野生型叶片中可溶性糖含量均大幅度下降。盐胁迫下突变体和野生型叶片细胞可溶性蛋白组分有明显的差异。mRNA差异显示结果表明,突变体有6个差异性的cDNA片段。

关键词： 小麦; 抗盐突变体; 脯氨酸; mRNA差异显示; 基因表达

Differences in the accumulation of some organic solutes and gene expression in leaves between the salt-tolerant mutant and the wild type of wheat under NaCl stress

Guo Fangqing, Zhou Jianming, Tang Zhangcheng

Shanghai Institute of Plant Physiology,Chinese Academy of Sciences.Shanghai 200032

Abstract

Proline content in leaves of the mutant increased more significantly than that of the wild type when exposed to NaCl stress. The content of proline increased for 72 h and then decreased in both types, but was higher in the mutant than in the wild type. When exposed to NaCl stress of 200 or 300 mmol/L for 96 h, the content of soluble sugars in leaves of the mutant maintained a higher level than that of the group without NaCl treatment, but that in the wild type became much lower than that of its control group.

Key words: Wheat;Salt tolerant mutant;Proline;mRNA differential display;Gene expression

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引用本文：

郭房庆, 周建明, 汤章城. NaCl胁迫下小麦突变体和野生型叶片中一些有机溶质累积和基因表达差异[J]. 生理学报 1999; 51 (3): .

Guo Fangqing, Zhou Jianming, Tang Zhangcheng. Differences in the accumulation of some organic solutes and gene expression in leaves between the salt-tolerant mutant and the wild type of wheat under NaCl stress. Acta Physiol Sin 1999; 51 (3): (in Chinese with English abstract).