光合细菌Rhodobacter sphaeroides glnB-lacZ融合子的构建与表达
梁莉, 朱美珍, 吴永强
中科院上海植物生理研究所.上海 200032
摘要
亚克隆了Rhodobacter sphaeroides glnB-lacZ启动子,以pMP220为载体构建成glnB-lacZ融合子。将glnB-lacZ、nifH-lacZ、nifA-lacZ分别导入R. sphaeroides谷氨酸合酶突变株gltB~(-)、gltD~(-)和野生型菌株中,分析了突变对固氮基因转录表达的影响。试验证明,在gltBD突变株中nifH的表达受阻遏,nifA表达水平很低。
关键词: 浑球红细菌; glnB; lacZ融合子; glt变种; 转录表达
Studies on the construction and expression of Rhodobacter sphaeroides glnB-lacZ fusion
Liang Li, Zhu Meizhen, Wu Yongqiang
Shanghai Institute of Plant Physiology,Chinese Academy of Sciences.Shanghai 200032
Abstract
Key words: Rhodobacter sphaeroides;glnB;lacZ fusion;glt mutant;Transcriptional expression
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引用本文:
梁莉, 朱美珍, 吴永强. 光合细菌Rhodobacter sphaeroides glnB-lacZ融合子的构建与表达[J]. 生理学报 1999; 51 (3): .
Liang Li, Zhu Meizhen, Wu Yongqiang. Studies on the construction and expression of Rhodobacter sphaeroides glnB-lacZ fusion. Acta Physiol Sin 1999; 51 (3): (in Chinese with English abstract).