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脑啡肽增强胶质细胞的神经营养作用与NO生成减少有关

魏光伟, 杜莅娜, 朱粹青, 唐崇仁, 曹小定, 吴根诚

上海医科大学神经生物学教研室.上海 200032

摘要

经脑啡脑处理的胶质细胞能使神经元的存活计数增加28%(P<0.05),单个神经元突起总长度增加11%(P<0.05),最长突起长度增加16%(P<0.05),GAP-43 mRNA的表达增加26%(P<0.05)。浓度为10~(-8)、10~(-10)mol/L的脑啡肽能明显抑制培养胶质细胞生成一氧化氮(P<0.05)。

关键词： 脑啡肽; 胶质细胞-神经元共培养; GAP-43; NO

Effect of enkephalin on glial neurotrophic function related to the reduction of nitric oxide production

Wei Guangwei, Du Lina, Zhu Cuiqing, Tang Chongren, Cao Xiaoding, Wu Gencheng

Department of Neurobiology,Shanghai Medical University.Shanghai 200032

Abstract

ME-treated glia enhanced neuronal survival by 28% (P<0.05), and increased total neurite length per neuron by 11% (P<0.05), while the expression of GAP-43 mRNA by 26% (P<0.05). The effect of ME on NO production of glia was also studied in rat cortical glial culture. The result showed that different concentrations of ME (10~(-12), 10~(-10), 10~(-8), 10~(-6)mol/L) inhibited glial NO production.

Key words: Methionine enkephalin;Glial neuronal coculture;GAP-43;NO

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引用本文：

魏光伟, 杜莅娜, 朱粹青, 唐崇仁, 曹小定, 吴根诚. 脑啡肽增强胶质细胞的神经营养作用与NO生成减少有关[J]. 生理学报 1999; 51 (3): .

Wei Guangwei, Du Lina, Zhu Cuiqing, Tang Chongren, Cao Xiaoding, Wu Gencheng. Effect of enkephalin on glial neurotrophic function related to the reduction of nitric oxide production. Acta Physiol Sin 1999; 51 (3): (in Chinese with English abstract).