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移植神经营养素-4基因工程细胞对脊髓前脚神经元的保护作用

侍坚, 曲伸, 路长林, 何小龙, 王成海

第二军医大学神经生物学教研室.上海 200433

摘要

用重组逆转录病毒介导的体外神经营养素-4(NT-4)基因转移方法,制备高表达NT-4基因工程细胞并移植大鼠坐骨神经离断处。尼氏染色和ChE染色的结果表明,移植NT-4基因工程细胞对外周神经损伤所造成的运动神经元的退变有显著的改善作用,而且这种作用可以维持三个月。

关键词： 神经营养素-4; 运动神经元; 外周神经损伤

Protection of spinal motorneurons of section sciatic nerve by transplantation of NT-4 genetically expression cells into the side of section

Shi Jian, Qu Shen, Lu Changlin, He Xiaolong, Wang Chenghai

Department of Neurobiology,The Second Military Medical University.Shanghai 200433

Abstract

The NT-4 genetically engineered cells were made by infecting L-6TG cell (a rat myoblast cell line) in vitro with a retroviral vector pN_(2)A containing the rat NT-4 cDNA. The bioactivities were determined by bioassay of PC12 cell survival rate. The rat with left sciatic nerve transaction was used as a model for treatment by implanting NT-4 genetically modified cell.

Key words: Neurotrophin-4;Motorneurons;Nerve injury

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引用本文：

侍坚, 曲伸, 路长林, 何小龙, 王成海. 移植神经营养素-4基因工程细胞对脊髓前脚神经元的保护作用[J]. 生理学报 1999; 51 (2): .

Shi Jian, Qu Shen, Lu Changlin, He Xiaolong, Wang Chenghai. Protection of spinal motorneurons of section sciatic nerve by transplantation of NT-4 genetically expression cells into the side of section. Acta Physiol Sin 1999; 51 (2): (in Chinese with English abstract).