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羧甲基壳聚糖对玉米籽粒氮代谢关键酶和种子贮藏蛋白含量的影响

师素云, 薛启汉, 刘蔼民, 练兴明, 王学臣

江苏省农业科学院农业生物遗传生理所.江苏,南京 210014；中国农业大学生物学院.北京 100094

摘要

以羧甲基壳聚糖(NCMC)处理玉米开花期果穗,发育中籽粒的谷氨酰胺合成酶(GS)、谷氨酸脱氢酶(GDH)及谷丙转氨酶(GPT)活性均明显增强,而蛋白水解酶活性下降。羧甲基壳聚糖处理离体玉米籽粒酶提取液,酶液中GDH活性明显提高。羧甲基壳聚糖处理玉米果穗,其发育籽粒中可溶性蛋白和成熟种子中贮藏蛋白含量明显提高。这都表明,羧甲基壳聚糖对玉米氮代谢、蛋白质合成与积累具有明显的生理调节作用。

关键词： 羧甲基壳聚糖; 氮代谢关键酶; 贮藏蛋白; 玉米

Effects of N-carboxymethylchitosan on N-metabolic key enzymes of grains and storage protein contents of seeds in maize

Shi Suyun, Xue Qihan, Liu Aimin, Lian Xingming, Wang Xuecheng

Institute of Agrobiological Genetics and Physiology,Jiangsu Academy of Agricultural Sciences.Nanjing 210014,Jiangsu；China

Abstract

The effects of N-carboxymethylchitosan on some of the important enzymes in nitrogen metabolism were studied by treating the ears of maize (Zea mays L.) with it at flowering stage. The activities of glutamine synthetase (GS), glutamate dehydrogenase (GDH), glutamic-pyruvic transaminase (GPT) and proteolytic enzyme were determined at different time after the treatment, and the contents of soluble protein, storage protein and components of storage proteins were also examined after harvest. It was found that activities of GS, GDH, and GPT in developing maize grains were increased remarkably by NCMC, which was also confirmed by in vitro enzyme reaction experiments, whereas proteolytic enzyme activities were decreased. Meanwhile, it was found that the contents of soluble protein in developing grains and storage protein in mature seeds were also increased remarkably. These results indicated that N-carboxymethylchitosan has a significant physiological regulatory action on N-metabolism and protein biosynthesis and accumulation in maize.

Key words: N-carboxymethylchitosan;N-metabolic key enzymes;Storage protein;Maize

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引用本文：

师素云, 薛启汉, 刘蔼民, 练兴明, 王学臣. 羧甲基壳聚糖对玉米籽粒氮代谢关键酶和种子贮藏蛋白含量的影响[J]. 生理学报 1999; 51 (2): .

Shi Suyun, Xue Qihan, Liu Aimin, Lian Xingming, Wang Xuecheng. Effects of N-carboxymethylchitosan on N-metabolic key enzymes of grains and storage protein contents of seeds in maize. Acta Physiol Sin 1999; 51 (2): (in Chinese with English abstract).