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超干红花种子抗老化作用及其机理

胡家恕, 朱诚, 曾广文, 郑光华

浙江大学华家池校区生物化学研究所.浙江,杭州 310029；浙江大学华家池校区植物生理教研室.浙江,杭州 310029；中科院植物研究所北京植物园.北京 100093

摘要

红花种子含水量(MC)降至5%以下,能显著提高抗老化劣变能力;在同等老化条件下,对照种子(MC=9.01%)活力已大幅度下降,而超干种子(MC=4.84%-1.88%)的活力保持不变。超干处理对红花种子脱氢酶、过氧化氢酶(CAT)及超氧物歧化酶(SOD)活性均无明显影响。经同等老化处理后,超干种子脱氢酶活性明显高于对照种子。在同等老化条件下,当对照种子CAT和SOD活性明显下降时,超干种子仍保持较高活性。红花种子在超干老化处理后,其胚中自由基水平相对地升高,超干种子经吸胀萌发前的回水处理,可使因老化导致的过多自由基及时得到清除,从而保证了超干种子能正常进入萌发状态。

关键词： 超干; 红花种子; 脱氢酶; 过氧化氢酶; 超氧物岐化酶; 自由基

Effect of aging-resistance of ultradried safflower seeds and its machanism

Hu Jiashu, Zhu Cheng, Zeng Guangwen, Zheng Guanghua

Institute of Biochemistry,Zhejiang University Hua Jia Chi Campus.Hangzhou 310029,Zhejiang；China；Beijing Botanical Garden,Chinese Academy of Sciences.Beijing 100093

Abstract

Experiments were conducted with safflower seeds rich in linoleic acid. Seed viability, seed vigor, aging-tolerance, morphological integrity of cells, lipid peroxidation and free radical levels in ultradried seeds were determined. The results showed that (1) Ultradrying treatment was not injurious to safflower seeds, it did not induce any significant change either in germination rate or seed vigor. Furthermore, our experimental data indicated that ultradried seeds became more tolerant under aging-accel erating treatment. (2) During ultradrying of safflower seeds, their content of free radicals increased, but decreased on rehydration before germination, which had a scavenging action. (3) The active-oxygen-scavenging system retained its capability during storage in an ultradry state.

Key words: Ultradry;Safflower seed;Dehydrogenase;Catalase;Superoxide dismutase;Free radical

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引用本文：

胡家恕, 朱诚, 曾广文, 郑光华. 超干红花种子抗老化作用及其机理[J]. 生理学报 1999; 51 (2): .

Hu Jiashu, Zhu Cheng, Zeng Guangwen, Zheng Guanghua. Effect of aging-resistance of ultradried safflower seeds and its machanism. Acta Physiol Sin 1999; 51 (2): (in Chinese with English abstract).