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适于青蒿芽生长和青蒿素积累的光、温和培养方式探讨

刘春朝, 王玉春, 康学真, 欧阳藩, 叶和春, 李国凤

中科院化工冶金研究所生化工程国家重点实验室.北京 100080；中科院植物研究所.北京 100093

摘要

适宜青蒿芽生长和青蒿素积累的光照强度约为3 000lx,照光时间为20h/d;芽生长和青蒿素积累的最适温度分别为25℃和30℃,通过先25℃(25d)后30℃(5d)的温度转变二步培养法可以提高青蒿素的产量;青蒿芽生长和青蒿素积累的最佳培养方式为非浸没低转速摇瓶培养。

关键词： 青蒿; 芽; 生长; 青蒿素

Investigation of light, temperature and cultivated modes on growth and artemisinin synthesis of Artemisia annua L. shoots

Liu Chunchao, Wang Yuchun, Kang Xuezhen, Ouyang Fan, Ye Hechun, Li Guofeng

State Key Laboratory of Biochemical Engineering,Institute of Chemical Metallurgy,Chinese Academy of Sciences.Beijing 100080；China

Abstract

The effects of light, temperature and cultivated modes on growth and artemisinin accumulation of Artemisia annua L. shoots (Fig.1) were investigated. The time course of shoots growth is shown in Fig.2. Figs.3 and 4 show that the artemisinin yield in shoots were enhanced under light irradiation length of 20 h/d with light intensity above 3 000 lx. The optimum temperatures for growth and artemisinin accumulation were 25℃ and 30℃ respectively (Fig.5). The artemisinin yield was improved in a bistaged temperature culture (25℃ for 25 days and then 30℃ for 5 days) (Fig.6). It was of beneficial to obtain the maximum artemisinin yield in shake flask culture with a shallow layer of liquid and low rotation (Fig.7).

Key words: Artemisia annua L.;Shoot;Growth;Artemisinin

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引用本文：

刘春朝, 王玉春, 康学真, 欧阳藩, 叶和春, 李国凤. 适于青蒿芽生长和青蒿素积累的光、温和培养方式探讨[J]. 生理学报 1999; 51 (2): .

Liu Chunchao, Wang Yuchun, Kang Xuezhen, Ouyang Fan, Ye Hechun, Li Guofeng. Investigation of light, temperature and cultivated modes on growth and artemisinin synthesis of Artemisia annua L. shoots. Acta Physiol Sin 1999; 51 (2): (in Chinese with English abstract).