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肝刺激因子对肝癌细胞增殖的调节作用

摘要

初断乳雄性SD大鼠的肝匀浆以超速离心和柱层析法分离纯化肝刺激因子(HSS),观察其对肝癌细胞增殖、细胞表皮生长因子(EGF)受体表达及受体磷酸化的影响。结果表明,HSS具有明显的促肝癌细胞分裂增殖能力,提高细胞周期中S期细胞所占比例。HSS促肝癌细胞增殖作用与其促EGF受体表达有关。

关键词： 肝刺激因子; 表皮生长因子; 肝细胞增殖

Regulatory effect of hepatic stimulator substance on the proliferation of human hepatoma cells

Abstract

The hepatic stimulator substance (HSS) was prepared from weanling rat livers by ultracentrifugation and Sephadex G-75 chromatography. The stimulatory effects of HSS on cell proliferation, EGF receptor expression and receptor tyrosine phosphorylation were investigated in serum-free cultured human hepatoma cell line SMMG-7721.

Key words: Hepatic stimulator substance;EGF receptor;Hepatocyte proliferation

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引用本文：

. 肝刺激因子对肝癌细胞增殖的调节作用[J]. 生理学报 1998; 50 (5): .

. Regulatory effect of hepatic stimulator substance on the proliferation of human hepatoma cells. Acta Physiol Sin 1998; 50 (5): (in Chinese with English abstract).