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盐胁迫对玉米根尖质膜上受钙激活的蛋白激酶的影响

陈武, 陈珈

中国农业大学生物学院.北京 100094

摘要

以0.2mol/L的NaCl对玉米根尖进行盐胁迫,导致质膜上一种受钙激活的蛋白激酶(该激酶表现为钙依赖蛋白激酶的特性)的活性迅速增加。盐胁迫至15min时激酶的活性达到最大,较对照高30%,以后逐渐降低,盐胁迫至50min时激酶活性仍略高于对照。10%PEG 6000处理玉米根尖也可诱导质膜上受钙激活的蛋白激酶活性增加。用外源ABA处理玉米根尖,未导致上述蛋白激酶活性的变化。放射自显影显示质膜上33kD和58kD两种蛋白可能是质膜受钙激活的蛋白激酶的内源底物。

关键词： 玉米根尖; 质膜; 钙; 钙激活的蛋白激酶; 盐胁迫; 信号转导; 钙依赖的蛋白激酶

Effect of salt stress on calcium-activated protein kinase associated with plasma membrane from corn (Zea mays L.) root tips

Chen Wu, Chen Jia

College of Biology,China Agricultural University.Beijing 100094

Abstract

NaCl 0.2mol/L treatment could induce quickly the activity of calcium-activated protein kinase, which showed the character of calcium-dependent protein kinase (CDPK), in plasma membrane (PM) from corn root tips. The activity reached its maximum after 15 min of salt stress. Then, the activity decreased gradually, remained higher than the control 50 min after the beginning of salt stress. Treatment with 10% PEG could also increase the activity of calcium-activated protein kinase. However, exogenous ABA did not change the activity of the calcium-activated protein kinase in PM. In gels assays of SDS-PAGE show that the proteins kinase contained only one polypeptide. It was shown by autoradiography that two proteins in PM with molecular weights of 33 kD and 58 kD may be the endogenous substrates of the calcium-activated protein kinase in PM.

Key words: Corn root tips;Plasma membrane;Calcium;Calcium-activated protein kinase;Salt stress;Signal transduction;Calcium-dependent protein knase

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引用本文：

陈武, 陈珈. 盐胁迫对玉米根尖质膜上受钙激活的蛋白激酶的影响[J]. 生理学报 1998; 50 (4): .

Chen Wu, Chen Jia. Effect of salt stress on calcium-activated protein kinase associated with plasma membrane from corn (Zea mays L.) root tips. Acta Physiol Sin 1998; 50 (4): (in Chinese with English abstract).