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花柱和花粉胞外钙调素对花粉萌发和花粉管伸长的影响

崔素娟, 王洪海, 马力耕, 孙大业

河北师范大学生物学系.河北,石家庄 050016；复旦大学遗传学研究所.上海 200433

摘要

以烟草为材料,通过半体内实验,就花柱和花粉胞外钙调素对花粉萌发和花粉管伸长的影响进行了观察。发现用EGTA及钙调素抗血清处理柱头或花粉均可抑制花粉在柱头上的萌发;向花柱引导组织中显微注射纯化钙调素可促进花粉管束伸长,而注射钙调素抗血清可抑制花粉管束伸长;同时证实玉米花柱和花粉细胞壁中均存在钙调素及钙调素结合蛋白,而且花粉和花柱细胞壁中钙调素结合蛋白的种类有差异。

关键词： 花柱; 花粉; 胞外钙调素; 花粉萌发; 花粉管伸长; 半体内

The effects of extracellular calmodulin of style and pollen on pollen germination and pollen tube growth

Cui Sujuan, Wang Honghai, Ma Ligeng, Sun Daye

Department of Biology,Hebei Normal University.Shijiazhuang 050016,Hebei；China

Abstract

The roles of extracellular calmodulin (Cam) of style and pollen in regulating pollen germination and tube growth were investigated by semivivo method. Pollen germination on stigma was inhibited when the stigma was pretreated with EGTA or anti-CaM serum. Pollen tube growth in style transmitting tissue was accelerated when the style was microinjected with exogenous purified CaM, but was inhibited by microinjection with anti-CaM serum. ELISA and biotinylated CaM overlay analyses showed that CaM and calmodulin-binding proteins (CaMBPs) were present in both pollen and style cell wall, and the kinds of CaMBPs in pollen cell wall were different from those in style cell wall.

Key words: Style;Polln;Extracellular calmodulin;Polln germination;Pollen tube growth; Semivivo

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引用本文：

崔素娟, 王洪海, 马力耕, 孙大业. 花柱和花粉胞外钙调素对花粉萌发和花粉管伸长的影响[J]. 生理学报 1998; 50 (4): .

Cui Sujuan, Wang Honghai, Ma Ligeng, Sun Daye. The effects of extracellular calmodulin of style and pollen on pollen germination and pollen tube growth. Acta Physiol Sin 1998; 50 (4): (in Chinese with English abstract).