菜豆重金属胁迫响应基因:cDNA克隆及其表达分析
柴团耀, 张玉秀, Burkard G
中国科学技术大学研究生院生物部.北京 100039;Institute de Biologie Moleculaire des Plantes du CNRS.Strasbourg 67084
摘要
通过差别筛选HgCl_(2)胁迫下的菜豆叶片cDNA库,分离出7组不同的cDNA克隆(Phaseolus vulgaris stress-related protein, PvSR1-7)。cDNA序列和同源性分析结果:PvSR1编码富含脯氨酸细胞壁蛋白(PRP),PvSR2和PvSR7编码新的HgCl_(2)胁迫相关蛋白,pVSR3编码脱水蛋白(dehydrin),PvSR4编码病原相关(PR)蛋白,PvSR5编码polyubiquitin,PvSR6编码DnaJ-like蛋白。HgCl_(2)胁迫可强烈地诱导PvSR2和PR蛋白基因的表达,并能提高PRP、dehydrin、DnaJ-like和polyubiquitin基因的转录水平。
Heavy metal-responsive genes in kidney bean: Cloning of cDNAs and gene expression analysis
Chai Tuanyao, Zhang Yuxiu, Burkard G
Department of Biology,Graduate School at Beijing,University of Science and Technology of China,Chinese Academy of Sciences.Beijing 100039;France
Abstract
The sequences of the cDNA inserts and homological analysis showed that they encode proline-rich protein (PvSR1), dehydrin (PvSR3), pathogenesis-related protein (PvSR4), polyubiquitin (PvSR5), DnaJ-like protein (PvSR6) and two new HgCl_(2)-stress-related proteins (PvSR2 and PvSR7). Northern blotting analysis revealed that the gene expression of PvSR2 and pathogenesis-related protein were strongly induced in leaf tissue by HgCl_(2) stress, the transcript levels of proline-rich protein, dehydrin, polyubiquitin, DnaJ-like protein and PvSR7 were greatly enhanced too. The various gene products might function to protect cell from heavy-metal stress.
Key words: Kidney bean (Phaseolus vulgaris L.;Heavy metal;DNA sequence and enalysis; Stress-related protein
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引用本文:
柴团耀, 张玉秀, Burkard G. 菜豆重金属胁迫响应基因:cDNA克隆及其表达分析[J]. 生理学报 1998; 50 (4): .
Chai Tuanyao, Zhang Yuxiu, Burkard G. Heavy metal-responsive genes in kidney bean: Cloning of cDNAs and gene expression analysis. Acta Physiol Sin 1998; 50 (4): (in Chinese with English abstract).