过刊浏览

菜豆重金属胁迫响应基因:cDNA克隆及其表达分析

柴团耀, 张玉秀, Burkard G

中国科学技术大学研究生院生物部.北京 100039；Institute de Biologie Moleculaire des Plantes du CNRS.Strasbourg 67084

摘要

通过差别筛选HgCl_(2)胁迫下的菜豆叶片cDNA库,分离出7组不同的cDNA克隆(Phaseolus vulgaris stress-related protein, PvSR1-7)。cDNA序列和同源性分析结果:PvSR1编码富含脯氨酸细胞壁蛋白(PRP),PvSR2和PvSR7编码新的HgCl_(2)胁迫相关蛋白,pVSR3编码脱水蛋白(dehydrin),PvSR4编码病原相关(PR)蛋白,PvSR5编码polyubiquitin,PvSR6编码DnaJ-like蛋白。HgCl_(2)胁迫可强烈地诱导PvSR2和PR蛋白基因的表达,并能提高PRP、dehydrin、DnaJ-like和polyubiquitin基因的转录水平。

关键词： 菜豆; 重金属; DNA序列分析; 胁迫蛋白

Heavy metal-responsive genes in kidney bean: Cloning of cDNAs and gene expression analysis

Chai Tuanyao, Zhang Yuxiu, Burkard G

Department of Biology,Graduate School at Beijing,University of Science and Technology of China,Chinese Academy of Sciences.Beijing 100039；France

Abstract

The sequences of the cDNA inserts and homological analysis showed that they encode proline-rich protein (PvSR1), dehydrin (PvSR3), pathogenesis-related protein (PvSR4), polyubiquitin (PvSR5), DnaJ-like protein (PvSR6) and two new HgCl_(2)-stress-related proteins (PvSR2 and PvSR7). Northern blotting analysis revealed that the gene expression of PvSR2 and pathogenesis-related protein were strongly induced in leaf tissue by HgCl_(2) stress, the transcript levels of proline-rich protein, dehydrin, polyubiquitin, DnaJ-like protein and PvSR7 were greatly enhanced too. The various gene products might function to protect cell from heavy-metal stress.

Key words: Kidney bean (Phaseolus vulgaris L.;Heavy metal;DNA sequence and enalysis; Stress-related protein

收稿日期：　　录用日期：

通讯作者：　　E-mail:

引用本文：

柴团耀, 张玉秀, Burkard G. 菜豆重金属胁迫响应基因:cDNA克隆及其表达分析[J]. 生理学报 1998; 50 (4): .

Chai Tuanyao, Zhang Yuxiu, Burkard G. Heavy metal-responsive genes in kidney bean: Cloning of cDNAs and gene expression analysis. Acta Physiol Sin 1998; 50 (4): (in Chinese with English abstract).